This study aimed to investigate the application of ultrasound guided puncture technique in blood collection in patients with severe trauma. The methods of this study is 93 patients with severe trauma were selected from the emergency room of the Second Affiliated Hospital of Zhejiang University from April 2020 to September 2,2020 and from October 2020 to March 2021, comparing the differences between nursing treatment efficiency and doctor satisfaction.

Standard process for blood collection using Ultrasonic Guided puncture.The control group used the traditional blood sample collection process, which was mainly based on personal clinical experience.

Statistical analysis using SPSS 25.0 statistical software. Observation index are time index of nursing treatment for patients with severe trauma: the time required for acquisition time, disposable puncture success rate, blood routine and blood type delivery time, rescue room stay time, etc and patient family and doctor satisfaction analysis: self-designed questionnaire, family satisfaction process, service time, service attitude, rescue technology, health education, including advice time, nursing quality, medical communication, teamwork, service attitude, evaluation levels: very satisfaction, satisfaction and dissatisfaction.

Inclusion Criteria:

• ① airway obstruction, need tracheal intubation or intubation;② breathing stop, respiratory distress or significant slowing down;③ circulatory system shows various shock signs④GCS≤8;⑤ body table visible head, torso, chest open injury or yoke chest

Exclusion Criteria:

• All patients who stop the rescue room trauma evaluation and treatment midway through hospital transfer and automatic discharge

All patients with severe trauma received in the emergency room of the Second Affiliated Hospital of Zhejiang University Medical College from April 2020 to March 2021