The aim of this study is to compare the endothelim-dependent and non-dependent vasodilatation between genders in the human gingiva.

Gender differences were observed in studies according to gingival microcirculation between physiological circumstances and during wound healing as well.

For blood flow measurement Laser Spackle Contrast Imaging is a reliable tool. It has good reproducibility, it doesn't get in touch with the measured surface, it's easy to use and it can measure on a bigger surface in same time.

Two wells were fabricated on the labial surface of the right lateral incisor (test tooth) and on the labial surface of the left central incisor (control tooth). These wells are opened towards the high-prermeability gingival sulcus, so the dropped solutions nitroglycerine vs physiological saline or nitroglycerine vs physiological saline can esasily penetrate to the gingival tissues. After applying the solutions, the blood flow was monitored fot 15 minutes.

Inclusion Criteria:

• general health
• minimum 5 mm keratinized gingiva at the upper front teeth
• good oral hygiene

Exclusion Criteria:

• pregnancy and breast-feeding
• smoking
• medication
• insufficient marginal integrity
• periodontitis
• caries