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Nano-based diagnostic tool can provide promising highly sensitive, specific biomarker for early detection and treatment of salivary gland tumours compared to non-conjugated biomarkers and in turn improves patient prognosis and outcome.
Cancer stem cells form a small subset of highly tumorigenic cells within the bulk of the tumours which mainly responsible for initiation, invasion, rapid growth, metastasis and therapeutic resistance in different types of human cancers. Nowadays, nanotechnology has increasing attention in multi-disciplinary research fields. Conjugated gold nanoparticles are widely used as biomarkers and bio-delivery vehicles in the medicine as well as early and advanced cancer detection and treatment. The current work aimed to introduce a novel diagnostic and prognostic approach in early detection of cancer stem cells in salivary gland tumours using gold nanoparticles conjugated to CD24 (CD24-Gold Nanocomposite).
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| CXPA group | carcinoma ex pleomorphic adenoma of major and minor salivary glands. |
| |
| PA group | Benign pleomorphic adenoma of major and minor salivary glands. |
| |
| Control group | normal controls obtained from border of excision biopsy of mucocele in the lip mucosa of healthy individuals. |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| CD24-Gold Nanocomposite expression using Real-time quantitative polymerase chain reaction | Diagnostic Test | new strategy where gold nanoparticles synthetized and added to CD24 primer forming CD24-Gold Nanocomposite to be used for detection of cancer stem cells |
| Measure | Description | Time Frame |
|---|---|---|
| Diagnostic potential of CD24-AuNC (index test) compared to non-conjugated CD24 (reference test) in determination of salivary gland tumours | we tested the differential expression of CD24-AuNC and non-conjugated CD24 biomarkers in all studied groups in order to identify the most sensitive and specific diagnostic biomarker to be used in detecting salivary gland tumours, | Done immediately following completion of assessment for eligibility of enrolment in the present study (enrolment took about 6 months) and confirmation of definite diagnosis |
| Measure | Description | Time Frame |
|---|---|---|
| Clinicopathological characteristics of the patients and their association with CD24-AuNC (Index test) and non-conjugated CD24 (Reference test) expressions | We investigated the relationship between both biomarkers' expression and clinicopathological characteristics of PA and CXPA patients such as age, gender, tumor site, tumor size (maximum diameter in mm), histopathological subtype, encapsulation, degree of invasion, facial nerve involvement and lymph node (LN) metastasis that may have direct relation with the tumor prognosis |
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Inclusion Criteria:
Exclusion Criteria:
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Patients presented to Oral and Maxillofacial Surgery Department, Faculty of Dentistry and Output Clinics of General Surgery Department, Faculty of Medicine, October 6 University, Giza, Egypt.
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| Name | Affiliation | Role |
|---|---|---|
| Amina F Farag, PhD | Faculty of Dentistry, October 6 University | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Faculty of Dentistry, October 6 University | Giza | 12511 | Egypt |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 37344887 | Derived | Kesharwani P, Ma R, Sang L, Fatima M, Sheikh A, Abourehab MAS, Gupta N, Chen ZS, Zhou Y. Gold nanoparticles and gold nanorods in the landscape of cancer therapy. Mol Cancer. 2023 Jun 21;22(1):98. doi: 10.1186/s12943-023-01798-8. | |
| 36089234 | Derived | Farag AF, Hassabou NF. CD24-gold nanocomposite as promising and sensitive biomarker for cancer stem cells in salivary gland tumors. Nanomedicine. 2022 Nov;46:102598. doi: 10.1016/j.nano.2022.102598. Epub 2022 Sep 8. |
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Briefly, a portion of surgically resected salivary gland tumour specimens was immersed immediately in liquid nitrogen, allowed to snap frozen and stored at -80 C (Frozen tissue) while the other portion was fixed in 10% buffered formalin solution and embedded in paraffin (Formalin-fixed paraffin-embedded tissue)
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| non-conjugated CD24 expression using Real-time quantitative polymerase chain reaction | Diagnostic Test | conventional strategy using non-conjugated CD24 expression to used for detection of cancer stem cells |
|
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| Done immediately following completion of assessment for eligibility of enrolment in the present study (enrolment took about 6 months) and confirmation of definite diagnosis |
| Prognostic significance of CD24-AuNC (index test) compared to non-conjugated CD24 (reference test) in assessing disease progression and/or patient survival | we assessed the disease progression and/or patient survival and examined its association with biomarkers expression | At regular intervals every 3 months for 24-months (follow-up period) |
| ID | Term |
|---|---|
| D006967 | Hypersensitivity |
| ID | Term |
|---|---|
| D007154 | Immune System Diseases |
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