Inflammatory Consequences of Crystalloids in Severely Burned Patients

Deciphering the Inflammatory Consequences of Crystalloids Used in the Resuscitation of Severely Burned Patients

This is a pilot study, but the investigators will also observe key immunological events with potential significance. The global objective is to study the inflammatory profiles of PlasmaLyte and Ringer's Lactate used in the initial massive fluid resuscitation of severely burned patients. On the long term, the investigators will identify the crystalloid that prevents hyperactivation of macrophages and death of severely burned patients.

The investigators have 4 specific objectives. First, the investigators will compare the activation profiles of macrophages and neutrophils in patients admitted to the severely burned unit with regards to the crystalloids used. Secondly, the investigators will assess the differences in the SOFA scores according to the crystalloids used. Thirdly, the investigators will evaluate the impact of crystalloids in the quality of skin grafts performed on severely burned patients. Lastly, with the help of a patient partner, the investigators will determine the right immunological terminology of the study for a non-scientific population.

Inclusion Criteria:

• admission to the CHUM
• more than 20% of burned area
• first blood withdrawal in the first 24 hours following burn

Exclusion Criteria:

• immunosuppression
• chemotherapy 6 months before admission
• radiotherapy 6 months before admission
• autoimmune diseases
• neoplasia
• pregnancy
• severe infections
• cardiac dysfunctions
• renal dysfunction
• hepatic dysfunctions
• Hepatitis C
• HIV