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B-cells ensure humoral immune response against antigens (Ag) thanks to their receptor (BCR). V(D)J rearrangement, somatic hypermutation, immunoglobulin (Ig) class switch and locus suicide recombination are mutational/recombinational processes targeting Ig loci influencing BCR expression. Study of these events is essential for B cell function analysis. Our project will provide the normal reference values using high throughput sequencing-based protocols.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Blood sample | Experimental |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Blood sample | Genetic | The blood samples will be collected from healthy volunteers : 7 tubes of 7 ml with anti-coagulant Héparine Lithium and 1 tube of 7ml with anti-coagulant EDTA |
| Measure | Description | Time Frame |
|---|---|---|
| Frequency of use of the V, D and J genes in VDJ rearrangements | through study completion, an average of 18 months |
| Measure | Description | Time Frame |
|---|---|---|
| Percentage of HyperMutation Somatic (SHM) in VDJ regions | through study completion, an average of 18 months | |
| Nature of HyperMutation Somatic (SHM) | transitions and transversions | through study completion, an average of 18 months |
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Inclusion Criteria:
healthy volunteers aged between 18 and 70
volunteers free from lymphoid hemopathy, immune deficiency and autoimmune disease.
3 categories : volunteers between 18 and 34 years of age, volunteers between 35 and 50 years of age, volunteers between 51 and 69 years of age.
Exclusion Criteria:
any recent vaccination (< 4 weeks)
tumoral pathology
lymphoïd hemopathy
immune deficiency
autoimmune disease
transplanted patients
inflammatory / systemic diseases
hypersensitivity or allergies
treatments likely to modify the immune response :
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Limoges University Hospital | Limoges | 87200 | France |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 37496419 | Result | Al Jamal I, Parquet M, Guiyedi K, Aoufouchi S, Le Guillou M, Rizzo D, Pollet J, Dupont M, Boulin M, Faumont N, Boutouil H, Jardin F, Ruminy P, El Hamel C, Lerat J, Al Hamaoui S, Makdissy N, Feuillard J, Gachard N, Peron S. IGH 3'RR recombination uncovers a non-germinal center imprint and c-MYC-dependent IGH rearrangement in unmutated chronic lymphocytic leukemia. Haematologica. 2024 Feb 1;109(2):466-478. doi: 10.3324/haematol.2023.282897. |
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| ID | Term |
|---|---|
| D001800 | Blood Specimen Collection |
| ID | Term |
|---|---|
| D013048 | Specimen Handling |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
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| Ig class switching (CSR) and recombination suicide of the IgH locus (LSR) junctions | number of CSR and LSR junctions | through study completion, an average of 18 months |
| Frequency of g class switching (CSR) and recombination suicide of the IgH locus (LSR) junctions | frequency of CSR and LSR junctions according to their structure | through study completion, an average of 18 months |
| D011677 | Punctures |
| D013514 | Surgical Procedures, Operative |
| D008919 | Investigative Techniques |