Subepithelial connective tissue grafts (CTG) are considered the gold standard procedure with proven clinical success when it comes to gingival augmentation aims to to improve periodontal health, which can enhance the long-term prognosis of teeth by obtaining soft tissue coverage of exposed root surfaces and/or augmentation the dimensions of gingival tissue. The procedure require harvesting tissue from a the palate, which increases the complexity of the procedure and patient discomfort. Complications after harvesting a graft would be pain, inflammation, bleeding, flap necrosis and infection in the donor site.

Inclusion Criteria:

• Patients with mucogingival defects scheduled for sub epithelial connective tissue graft
• Age at least 18 years.
• Not having any systemic disease that could compromise wound healing
• No periodontal surgery on the experimental sites.
• No smoking,
• No pregnancy or lactation

Exclusion Criteria:

• Patients with coagulation disorders
• patients on corticosteroids, with uncontrolled diabetes, or with any systemic disease that precluded periodontal surgery
• patients with a history of contact dermatitis to formaldehyde
• Loss of maxillary premolars and molars
• Medications or antibiotics used in the previous 6 months
• Pregnancy or lactation
• Smoking