Development of a Novel Method to Measure Insulin Sensitivity in Humans: A Pilot Study

Development of a Novel Method to Measure Insulin Sensitivity in Humans: A Pilot Study

This study will determine whether the ratio between 24h C-peptide urinary excretion rate and average 24h circulating glucose represent a good correlate of what is measured by the gold standard, i.e. M (glucose disposal rate) from a euglycemic-hyperinsulinemic clamp

Inclusion Criteria:

• Healthy male or female
• Age 18-65 y
• BMI between 20 kg/m2 and 35 kg/m2 (inclusive)
• Are willing to consume pre-prepared meals
• Have completed a high-dose (≥80 mIU/m2/min) euglycemic-hyperinsulinemic clamp during the last five years at Pennington Biomedical. The data from the previous PBRC clamp will be pulled and used in conjunction with data from this study.
• Willing to have blood and urine stored for future use

Exclusion Criteria:

• Major lifestyle changes since the euglycemic-hyperinsulinemic clamp was performed (i.e. gain/lost weight, stopped smoking, began/stop exercise).
• Unstable weight in the last 3 months [gain or loss >10 lb (or 4.5 kg)]
• Diagnosed with diabetes
• Untreated hypertension and average screening blood pressure >140/90 mmHg
• Previous bariatric surgery (or other surgeries) for obesity or weight loss
• Chronic use of medications affecting metabolism or sleep*
• History of neurological disease
• History of cardiovascular disease, or other chronic diseases, that might affect pancreatic or glucose metabolism.
• Pregnant, planning to become pregnant, or breastfeeding
• Adherence to special restrained diets (e.g., low-CHO, low-fat, or vegetarian/vegan diets) over the last 3 months. *Sporadic use of these medications is fine (however, enrollment will depend on a case-by-case basis). If taking sporadically, participants should not be taking the medication for 1-month prior to the first visit.