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| ID | Type | Description | Link |
|---|---|---|---|
| 2020-A02879-30 | Other Identifier | ANSM |
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CIDP, a rare disorder affecting young adults, causes gradual weakness of the limbs, areflexia and impaired sensory function. New CIDP phenotypes without antibodies but with modified cell profiles have been described. Treatments include corticotherapy, IVIg and plasmapheresis but the latter's action mechanisms remain unclear. Plasmapheresis supposedly removes toxic agents like antibodies from plasma but it is uncertain whether it has an immune-modulating effect. Also, the refining mechanisms of the two main plasmapheresis techniques - single plasma exchange and double filtration plasmapheresis (DFPP) - are different and unclear. This study aims to compare the evolution of peripheral lymphocyte profiles in patients with CIDP according to their treatment (single centrifugation plasmapheresis or DFPP) to better grasp the action mechanisms of both techniques.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Group A | Experimental | Patients alternating single plasma exchange followed by double filtration plasmapheresis etc. |
|
| Group B | Experimental | Patients alternating double filtration plasmapheresis followed by single plasma exchange etc. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Blood sampling | Biological | Blood sampling before and after each type of cycle, Single Plasma Exchange or Double Filtration Plasmapheresis |
|
| Measure | Description | Time Frame |
|---|---|---|
| Cycle 1, Group A : TH1 cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 0, Time 0 |
| Cycle 1, Group A : TH1 cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 0, Hour 3 |
| Cycle 1, Group A : TH1 cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 7 -10 |
| Cycle 1, Group A : TH17 cells BEFORE first single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 peripheral lymphocyte cells will be measured in %. | Day 0, Time 0 |
| Cycle 1, Group A : TH17 cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 peripheral lymphocyte cells will be measured in %. | Day 0, Hour 3 |
| Cycle 1, Group A : TH17 cells 7 - 10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 peripheral lymphocyte cells will be measured in %. | Day 7 - 10 |
| Measure | Description | Time Frame |
|---|---|---|
| Cycle 1, Group A : T4 lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 1, Group A : T4-DR+ lymphocytes BEFORE single plasma exchange |
| Measure | Description | Time Frame |
|---|---|---|
| Group A, Cycle 1 : Link between variations in lymphocyte sub-populations and the clinical evolution . | All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle. | Day 7-10 after the end of Cycle 1 (each cycle lasts 3 hours) |
Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Olivier MORANNE | Nîmes | France |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 35931957 | Background | Moranne O, Ion IM, Cezar R, Messikh Z, Prelipcean C, Chkair S, Thouvenot E, Tran TA, Corbeau P, Chevallier T. Protocol of comparison of the effects of single plasma exchange and double filtration plasmapheresis on peripheral lymphocyte phenotypes in patients with Chronic Inflammatory Demyelinating Polyradiculoneuropathy: a monocentric prospective study with single-case experimental design. BMC Neurol. 2022 Aug 5;22(1):293. doi: 10.1186/s12883-022-02816-w. |
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Proof-of-concept, monocentric, prospective, Single-Case Experimental Design
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Randomisation to one of two treatments before alternation.
| Cycle 1, Group A : T-reg cells BEFORE first single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %. | Day 0, Hour 0 |
| Cycle 1, Group A : T-reg cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %. | Day 0, Hour 3 |
| Cycle 1, Group A : T-reg cells 7 - 10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %. | Day 7 - 10 |
| Cycle 1, Group B : TH1 cells BEFORE first double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 0, Hour 0 |
| Cycle 1, Group B : TH1 cells AFTER the first double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 0, Hour 3 |
| Cycle 1, Group B : TH1 cells 7-10 days AFTER the first double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 7 - 10 |
| Cycle 1, Group B : TH17 cells BEFORE the first double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %. | Day0, Hour 0 |
| Cycle 1, Group B : TH17 cells AFTER the first double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %. | Day 0, Hour 3 |
| Cycle 1, Group B : T17 cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %. | Day 7-10 |
| Cycle 1, Group B : T-reg cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %. | Day 0, Hour 0 |
| Cycle 1, Group B : T-reg cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %. | Day 0, Hour 3 |
| Cycle 1, Group B : T-reg cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %. | Day 7 - 10 |
| Cycle 2, Group A: TH1 cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 cells will be measured in %. | Day 0, Hour 0 |
| Cycle 2, Group A: TH1 cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 cells will be measured in %. | Day 0, Hour 3 |
| Cycle 2, Group A: TH1 cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 cells will be measured in %. | Day 7 - 10 |
| Cycle 2, Group A: TH17 cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %. | Day 0, Hour 0 |
| Cycle 2, Group A: TH17 cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %. | Day 0, Hour 3 |
| Cycle 2, Group A: TH17 cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %. | Day 7-10 |
| Cycle 2, Group A: T-reg cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %. | Day 0, Hour 0 |
| Cycle 2, Group A: T-reg cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %. | Day 0, Hour 3 |
| Cycle 2, Group A: T-reg cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %. | Day 7-10 |
| Cycle 2, Group B: TH1 cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 cells will be measured in %. | Day 0, Hour 0 |
| Cycle 2, Group B: TH1 cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 cells will be measured in %. | Day 0, Hour 3 |
| Cycle 2, Group B: TH1 cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 cells will be measured in %. | Day 7-10 |
| Cycle 2, Group B: T17 cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T17 cells will be measured in %. | Day 0, Hour 0 |
| Cycle 2, Group B: TH17 cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T17 cells will be measured in %. | Day 0, Hour 3 |
| Cycle 2, Group B: TH17 cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %. | Day 7-10 |
| Cycle 2, Group B: T-reg cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %. | Day 0, Hour 0 |
| Cycle 2, Group B: T-reg cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %. | Day 0, Hour 3 |
| Cycle 2, Group B: T-reg cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %. | Day 7-10 |
| Cycle 3, Group A: TH1 cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 1, Hour 1 |
| Cycle 3, Group A: TH1 cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 1, Hour 3 |
| Cycle 3, Group A: T1 cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 7 - 10 |
| Cycle 3, Group A: TH17 cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %. | Day 1, Hour 1 |
| Cycle 3, Group A: TH17 cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %. | Day 1, Hour 3 |
| Cycle 3, Group A: TH17 cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %. | Day 7 - 10 |
| Cycle 3, Group A: T-reg cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %. | Day 0, Hour 0 |
| Cycle 3, Group A: T-reg cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %. | Day 0, Hour 3 |
| Cycle 3, Group A: T-reg cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %. | Day 7-10 |
| Cycle 3, Group B: TH1 cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 0, Hour 0 |
| Cycle 3, Group B: TH1 cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 0, Hour 3 |
| Cycle 3, Group B: TH1 cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 7-10 |
| Cycle 3, Group B: TH17 cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %. | Day 0, Hour 0 |
| Cycle 3, Group B: TH17 cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %. | Day 0, Hour 3 |
| Cycle 3, Group B: TH17 cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %. | Day 7-10 |
| Cycle 3, Group B: T-reg cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %. | Day 0, Hour 0 |
| Cycle 3, Group B: T-reg cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %. | Day 0, Hour 3 |
| Cycle 3, Group B: T-reg cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %. | Day 7-10 |
| Cycle 4, Group A: T1 cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T1 peripheral lymphocyte cells will be measured in %. | Day 0, Hour 0 |
| Cycle 4, Group A: T1 cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T1 peripheral lymphocyte cells will be measured in %. | Day 0, Hour 3 |
| Cycle 4, Group A: TH1 cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 7 - 10 |
| Cycle 4, Group A: TH17 cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %. | Day 0, Hour 0 |
| Cycle 4, Group A: TH17 cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %. | Day 0, Hour 3 |
| Cycle 4, Group A: TH17 cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 peripheral lymphocyte cells will be measured in %. | Day 7 - 10 |
| Cycle 4, Group A: T-reg cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %. | Day 0, Hour 0 |
| Cycle 4, Group A: T-reg cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %. | Day 0, Hour 3 |
| Cycle 4, Group A: T-reg cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg peripheral lymphocyte cells will be measured in %. | Day 7 - 10 |
| Cycle 4, Group B : TH1 cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 0, Hour 0 |
| Cycle 4, Group B : TH1 cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 0, Hour 3 |
| Cycle 4, Group B : TH1 cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 7 -10 |
| Cycle 4, Group B : TH17 cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %. | Day 0, Hour 0 |
| Cycle 4, Group B : TH17 cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %. | Day 0, Hour 3 |
| Cycle 4, Group B : T17 cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T1 peripheral lymphocyte cells will be measured in %. | Day 7 -10 |
| Cycle 4, Group B : T-reg cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %. | Day 0, Hour 0 |
| Cycle 4, Group B : T-reg cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %. | Day 0, Hour 3 |
| Cycle 4, Group B : T-reg cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %. | Day 7 - 10 |
| Cycle 5, Group A : TH1 cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 0, Hour 0 |
| Cycle 5, Group A : TH1 cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 0, Hour 3 |
| Cycle 5, Group A : TH1 cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 cells will be measured in %. | Day 7 - 10 |
| Cycle 5, Group A : TH17 cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %. | Day 0, Hour 0 |
| Cycle 5, Group A : TH17 cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %. | Day 0, Hour 3 |
| Cycle 5, Group A : TH17 cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %. | Day 7 - 10 |
| Cycle 5, Group A : T-reg cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %. | Day 0, Hour 0 |
| Cycle 5, Group A : T-reg cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %. | Day 0, Hour 3 |
| Cycle 5, Group A : T-reg cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %. | Day 7 - 10 |
| Cycle 5, Group B : TH1 cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 0, Hour 0 |
| Cycle 5, Group B : TH1 cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 0, Hour 3 |
| Cycle 5, Group B : TH1 cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 7-10 |
| Cycle 5, Group B : TH17 cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %. | Day 0, Hour 0 |
| Cycle 5, Group B : TH17 cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %. | Day 0, Hour 3 |
| Cycle 5, Group B : TH17 cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %. | Day 7-10 |
| Cycle 5, Group B : T-reg cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %. | Day 0, Hour 0 |
| Cycle 5, Group B : T-reg cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %. | Day 0, Hour 3 |
| Cycle 5, Group B : T-reg cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %. | Day 7-10 |
| Cycle 6, Group A : TH1 cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 0 Hour 0 |
| Cycle 6, Group A : TH1 cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 0 Hour 3 |
| Cycle 6, Group A : TH1 cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 7-10 |
| Cycle 6, Group A : TH17 cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %. | Day 0 Hour 0 |
| Cycle 6, Group A : TH17 cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %. | Day 0 Hour 3 |
| Cycle 6, Group A : TH17 cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %. | Day 7-10 |
| Cycle 6, Group A : T-reg cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %. | Day 0 Hour 0 |
| Cycle 6, Group A : T-reg cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %. | Day 0 Hour 3 |
| Cycle 6, Group A : T-reg cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %. | Day 7-10 |
| Cycle 6, Group B : TH1 cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 0 Hour 0 |
| Cycle 6, Group B : TH1 cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 0 Hour 3 |
| Cycle 6, Group B : TH1 cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %. | Day 7-10 |
| Cycle 6, Group B : TH17 cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %. | Day 0 Hour 0 |
| Cycle 6, Group B : TH17 cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %. | Day 0 Hour 3 |
| Cycle 6, Group B : TH17 cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %. | Day 7-10 |
| Cycle 6, Group B : T-reg cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %. | Day 0 Hour 0 |
| Cycle 6, Group B : T-reg cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %. | Day 0 Hour 3 |
| Cycle 6, Group B : T-reg cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %. | Day 7-10 |
5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. |
| Day 0 Hour 0 |
| Cycle 1, Group A : T8 lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 1, Group A : T8 DR+ lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 1, Group A : B lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 1, Group A : CD45RA+CCR7+ cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 1, Group A : CD45RA-CCR7+ cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 1, Group A : CD45RA-CCR7- cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 1, Group A : CCR6-CXCR3- cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 1, Group A : T4 lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 1, Group A : T4-DR+ lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 1, Group A : T8 lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 1, Group A : T8 DR+ lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 1, Group A : B lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 1, Group A : CD45RA+CCR7+ cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 1, Group A : CD45RA-CCR7+ cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 1, Group A : CD45RA-CCR7- cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 1, Group A : CR6-CXCR3- cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 1, Group A : T4 lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 1, Group A : T4-DR+ lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 1, Group A : T8 lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 1, Group A : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 1, Group A : B lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 1, Group A : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 1, Group A : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 1, Group A : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 1, Group A : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 1, Group B: T4 lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 1, Group B: T4 DR+ lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 1, Group B: T8 lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 1, Group B: T8 DR+ lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 1, Group B: B lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 1, Group B: CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 1, Group B: CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 1, Group B: CD45RA-CCR7- cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 1, Group B: CCR6-CXCR3- cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 1, Group B:T4 lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 1, Group B:T4 DR+ lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 1, Group B:T8 lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 1, Group B:T8 DR+ lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 1, Group B: B lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 1, Group B: CD45RA+CCR7+ cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 1, Group B: CD45RA-CCR7+ cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 1, Group B: CD45RA-CCR7- cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 1, Group B: CCR6-CXCR3- cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 1, Group B: T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 1, Group B: T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 1, Group B: T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 1, Group B: T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 1, Group B: B lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 1, Group B: CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 1, Group B: CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 1, Group B: CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 1, Group B: CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 2, Group A : T4 lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 2, Group A : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 2, Group A : T8 lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 2, Group A : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 2, Group A : B lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage. | Day 0 Hour 0 |
| Cycle 2, Group A : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage. | Day 0 Hour 0 |
| Cycle 2, Group A : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage. | Day 0 Hour 0 |
| Cycle 2, Group A : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage. | Day 0 Hour 0 |
| Cycle 2, Group A : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage. | Day 0 Hour 0 |
| Cycle 2, Group A : T4 lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage. | Day 0 Hour 3 |
| Cycle 2, Group A : T4 DR+ lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 2, Group A : T8 lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 2, Group A : T8 DR+ lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 2, Group A : B lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 2, Group A : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 2, Group A : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 2, Group A : CD45RA-CCR7- cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 2, Group A : CCR6-CXCR3- cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 2, Group A : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7 - 10 |
| Cycle 2, Group A : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7 - 10 |
| Cycle 2, Group A : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7 - 10 |
| Cycle 2, Group A : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7 - 10 |
| Cycle 2, Group A : B lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7 - 10 |
| Cycle 2, Group A : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7 - 10 |
| Cycle 2, Group A : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7 - 10 |
| Cycle 2, Group A : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7 - 10 |
| Cycle 2, Group A : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7 - 10 |
| Cycle 2, Group B : T4 lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 2, Group B : T4 DR+ lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 2, Group B : T8 lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 2, Group B : T8 DR+ lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 2, Group B : B lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 2, Group B : CD45RA+CCR7+ cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 2, Group B : CD45RA-CCR7+ cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 2, Group B : CD45RA-CCR7- cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 2, Group B : CCR6-CXCR3- cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 2, Group B : T4 lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 2, Group B : T4 DR+ lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 2, Group B : T8 lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 2, Group B : T8 DR+ lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 2, Group B : B lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 2, Group B : CD45RA+CCR7+ cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 2, Group B : CD45RA-CCR7+ cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 2, Group B : CD45RA-CCR7- cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 2, Group B : CCR6-CXCR3- cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 2, Group B : T4 lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 2, Group B : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 2, Group B : T8 lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 2, Group B : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 2, Group B : B lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 2, Group B : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 2, Group B : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 2, Group B : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured a percentage. | Day 7-10 |
| Cycle 2, Group B : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 3, Group A : T4 lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 3, Group A : T4 DR+ lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 3, Group A : T8 lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 3, Group A : T8 DR+ lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 3, Group A : B lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 3, Group A : CC45RA+CCR7+ cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 3, Group A : CC45RA-CCR7+ cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 3, Group A : CC45RA-CCR7- cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 3, Group A : CCR6-CXCR3- cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 3, Group A : T4 lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 3, Group A : T4 DR+ lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 3, Group A : T8 lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 3, Group A : T8 DR+ lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 3, Group A : B lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 3, Group A : CD45RA+CCR7+ cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 3, Group A : CD45RA-CCR7+ cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 3, Group A : CD45RA-CCR7- cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 3, Group A : CCR6-CXCR3- cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 3, Group A : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 3, Group A : T4 lymphocytes 7 -10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 3, Group A : T8 lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 3, Group A : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 3, Group A : B lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 3, Group A : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 3, Group A : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 3, Group A : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 3, Group A : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 3, Group B : T4 lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 3, Group B : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 3, Group B : T8 lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 3, Group B : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 3, Group B : B lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 3, Group B : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 3, Group B : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 3, Group B : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 3, Group B : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 3, Group B : T4 lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 3, Group B : T4 DR+ lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 3, Group B : T8 lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 3, Group B : T8 DR+ lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 3, Group B : B lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 3, Group B : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 3, Group B : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 3, Group B : CD45RA-CCR7- cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 3, Group B : CCR6-CXCR3- cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 3, Group B : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 3, Group B : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 3, Group B : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 3, Group B : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 3, Group B : B lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 3, Group B : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 3, Group B : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 3, Group B : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 3, Group B : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 4, Group A : T4 lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 4, Group A : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 4, Group A : T8 lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 4, Group A : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 4, Group A : B lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 4, Group A : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 4, Group A : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 4, Group A : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 4, Group A : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 4, Group A : T4 lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 4, Group A : T4 DR+ lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 4, Group A : T8 lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 4, Group A : T8 DR+ lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 4, Group A : B lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 4, Group A : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 4, Group A : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 4, Group A : CD45RA-CCR7- cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 4, Group A : CCR6-CXCR3- cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 4, Group A : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 4, Group A : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 4, Group A : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 4, Group A : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 4, Group A : B lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 4, Group A : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 4, Group A : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 4, Group A : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 4, Group A : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 4, Group B : T4 lymphocytes BEFORE plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 4, Group B : T4 DR+ lymphocytes BEFORE plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 4, Group B : T8 lymphocytes BEFORE plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 4, Group B : T8 DR+ lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 4, Group B : B lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 4, Group B : CD45RA+CCR7+ cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 4, Group B : CD45RA-CCR7+ cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 4, Group B : CD45RA-CCR7- cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 4, Group B : CCR6-CXCR3- cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 0 |
| Cycle 4, Group B : T4 lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 4, Group B : T4 DR+ lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 4, Group B : T8 lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 4, Group B : T8 DR+ lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 4, Group B : B lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 4, Group B : CD45RA+CCR7+ cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 4, Group B : CD45RA-CCR7+ cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 4, Group B : CD45RA-CCR7- cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 4, Group B : CCR6-CXCR3- cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0 Hour 3 |
| Cycle 4, Group B : T4 lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 4, Group B : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 4, Group B : T8 lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 4, Group B : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 4, Group B : B lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 4, Group B : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 4, Group B : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 4, Group B : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 4, Group B : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 5, Group A : T4 lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 5, Group A : T4 DR+ lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 5, Group A : T8 lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 5, Group A : T8 DR+ lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 5, Group A : B lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 5, Group A : CD45RA+CCR7+ cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 5, Group A : CD45RA-CCR7+ cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 5, Group A : CD45RA-CCR7- cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 5, Group A : CCR6-CXCR3- cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 5, Group A : T4 lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 5, Group A : T4 DR+ lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 5, Group A : T8 lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 5, Group A : T8 DR+ lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 5, Group A : B lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 5, Group A : CD45RA+CCR7+ cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 5, Group A : CD45RA-CCR7+ cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 5, Group A : CD45RA-CCR7- cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 5, Group A : CCR6-CXCR3- cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 5, Group A : T4 lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 5, Group A : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 5, Group A : T8 lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 5, Group A : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 5, Group A : B lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 5, Group A : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 5, Group A : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 5, Group A : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 5, Group A : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 5, Group B : T4 lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 5, Group B : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 5, Group B : T8 lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 5, Group B : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 5, Group B : B lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 5, Group B : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 5, Group B : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 5, Group B : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 5, Group B : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 5, Group B : T4 lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 5, Group B : T4 DR+ lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 5, Group B : T8 lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 5, Group B : T8 DR+ lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 5, Group B : B lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 5, Group B : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 5, Group B : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 5, Group B : CD45RA-CCR7- cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 5, Group B : CCR6-CXCR3- cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 5, Group B : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 5, Group B : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 5, Group B : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 5, Group B : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 5, Group B : B lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 5, Group B : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 5, Group B : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 5, Group B : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 5, Group B : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 6, Group A : T4 lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 6, Group A : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 6, Group A : T8 lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 6, Group A : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 6, Group A : B lymphocytes BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 6, Group A : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 6, Group A : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 6, Group A : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 6, Group A : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 0 |
| Cycle 6, Group A : T4 lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 6, Group A : T4 DR+ lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 6, Group A : T8 lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 6, Group A : T8 DR+ lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 6, Group A : B lymphocytes AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 6, Group A : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 6, Group A : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 6, Group A : CD45RA-CCR7- cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 6, Group A : CCR6-CXCR3- cells AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, Hour 3 |
| Cycle 6, Group A : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 6, Group A : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 6, Group A : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 6, Group A : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 6, Group A : B lymphocytes 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 6, Group A : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 6, Group A : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 6, Group A : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 6, Group A : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis | 5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 6, Group B : T4 lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, H0 |
| Cycle 6, Group B : T4 DR+ lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, H0 |
| Cycle 6, Group B : T8 lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, H0 |
| Cycle 6, Group B : T8 DR+ lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, H0 |
| Cycle 6, Group B : B lymphocytes BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, H0 |
| Cycle 6, Group B : CD45RA+CCR7+ cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, H0 |
| Cycle 6, Group B : CD45RA-CCR7+ cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, H0 |
| Cycle 6, Group B : CD45RA-CCR7- cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, H0 |
| Cycle 6, Group B : CCR6-CXCR3- cells BEFORE single plasma exchange | 5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, H0 |
| Cycle 6, Group B : T4 lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, H3 |
| Cycle 6, Group B : T4 DR+ lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, H3 |
| Cycle 6, Group B : T8 lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, H3 |
| Cycle 6, Group B : T8 DR+ lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, H3 |
| Cycle 6, Group B : B lymphocytes AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, H3 |
| Cycle 6, Group B : CD45RA+CCR7+ cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, H3 |
| Cycle 6, Group B : CD45RA-CCR7+ cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, H3 |
| Cycle 6, Group B : CD45RA-CCR7- cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, H3 |
| Cycle 6, Group B : CCR6-CXCR3- cells AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 0, H3 |
| Cycle 6, Group B : T4 lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 6, Group B : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 6, Group B : T8 lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 6, Group B : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 6, Group B : B lymphocytes 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 6, Group B : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 6, Group B : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 6, Group B : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Cycle 6, Group B : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange | 5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage. | Day 7-10 |
| Group B, Cycle 1 : Link between variations in lymphocyte sub-populations and the clinical evolution . | All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle. | Day 7-10 after the end of Cycle 1 (each cycle lasts 3 hours) |
| Group A, Cycle 2 : Link between variations in lymphocyte sub-populations and the clinical evolution . | All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle. | Day 7-10 after the end of Cycle 2 (each cycle lasts 3 hours) |
| Group B, Cycle 2 : Link between variations in lymphocyte sub-populations and the clinical evolution . | All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle. | Day 7-10 after the end of Cycle 2 (each cycle lasts 3 hours) |
| Group A, Cycle 3 : Link between variations in lymphocyte sub-populations and the clinical evolution . | All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle. | Day 7-10 after the end of Cycle 3 (each cycle lasts 3 hours) |
| Group B, Cycle 3 : Link between variations in lymphocyte sub-populations and the clinical evolution . | All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle. | Day 7-10 after the end of Cycle 3 (each cycle lasts 3 hours) |
| Group A, Cycle 4 : Link between variations in lymphocyte sub-populations and the clinical evolution . | All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle. | Day 7-10 after the end of Cycle 4 (each cycle lasts 3 hours) |
| Group B, Cycle 4 : Link between variations in lymphocyte sub-populations and the clinical evolution . | All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle. | Day 7-10 after the end of Cycle 4 (each cycle lasts 3 hours) |
| Group A, Cycle 5 : Link between variations in lymphocyte sub-populations and the clinical evolution . | All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle. | Day 7-10 after the end of Cycle 5 (each cycle lasts 3 hours) |
| Group B, Cycle 5 : Link between variations in lymphocyte sub-populations and the clinical evolution . | All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle. | Day 7-10 after the end of Cycle 5 (each cycle lasts 3 hours) |
| Group A, Cycle 6 : Link between variations in lymphocyte sub-populations and the clinical evolution . | All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle. | Day 7-10 after the end of Cycle 6 (each cycle lasts 3 hours) |
| Group B, Cycle 6 : Link between variations in lymphocyte sub-populations and the clinical evolution . | All the above primary and secondary outcome measures will be compared with clinical information in the patient file in order to determine a link between these variations at the end of each treatment cycle. | Day 7-10 after the end of Cycle 6 (each cycle lasts 3 hours) |
| Group A, Cycle 1 : Cost of single plasma exchange to the health institution. | The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles. | Day 7-10 after the end of Cycle 1 (each cycle lasts 3 hours) |
| Group B, Cycle 1 : Cost of double filtration plasmapheresis to the health institution. | The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles. | Day 7-10 after the end of Cycle 1 (each cycle lasts 3 hours) |
| Group A, Cycle 2 : Cost of double filtration plasmapheresis to the health institution. | The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles. | Day 7-10 after the end of Cycle 2 (each cycle lasts 3 hours) |
| Group B, Cycle 2 : Cost of single plasma exchange to the health institution. | The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles. | Day 7-10 after the end of Cycle 2 (each cycle lasts 3 hours) |
| Group A, Cycle 3 : Cost of single plasma exchange to the health institution. | The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles. | Day 7-10 after the end of Cycle 3 (each cycle lasts 3 hours) |
| Group B, Cycle 3 : Cost of double filtration plasmapheresis to the health institution. | The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles. | Day 7-10 after the end of Cycle 3 (each cycle lasts 3 hours) |
| Group A, Cycle 4 : Cost of double filtration plasmapheresis to the health institution. | The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles. | Day 7-10 after the end of Cycle 4 (each cycle lasts 3 hours) |
| Group B, Cycle 4 : Cost of single plasma exchange to the health institution. | The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles. | Day 7-10 after the end of Cycle 4 (each cycle lasts 3 hours) |
| Group A, Cycle 5 : Cost of single plasma exchange to the health institution. | The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles. | Day 7-10 after the end of Cycle 5 (each cycle lasts 3 hours) |
| Group B, Cycle 5 : Cost of double filtration to the health institution. | The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles. | Day 7-10 after the end of Cycle 5 (each cycle lasts 3 hours) |
| Group A, Cycle 6 : Cost of double filtration to the health institution. | The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles. | Day 7-10 after the end of Cycle 6 (each cycle lasts 3 hours) |
| Group B, Cycle 6 : Cost of double filtration to the health institution. | The cost of each cycle will be recorded in Euros in the electronic clinical research file and the average amount will be calculated from this information at the end of 6 cycles. | Day 7-10 after the end of Cycle 6 (each cycle lasts 3 hours) |
| Constitution of a biological resource centre using the blood samples taken in view of future research, especially immunological phenotyping. | After all the above analyses, the unused aliquots from the samples taken (bottom of tubes) will be conserved and indexed. | Day 0, Hour 0 |
| Constitution of a biological resource centre using the blood samples taken in view of future research, especially immunological phenotyping. | After all the above analyses, the unused aliquots from the samples taken (bottom of tubes) will be conserved and indexed. | Day 0, Hour 3 |
| Constitution of a biological resource centre using the blood samples taken in view of future research, especially immunological phenotyping. | After all the above analyses, the unused aliquots from the samples taken (bottom of tubes) will be conserved and indexed. | Day 7-10 |
| Compare lymphocyte phenotype between patients with PIDC and control subjects. | Significant difference in Th1/Th2 ratio between PIDC patients and control subjects. | Day 0 |
| ID | Term |
|---|---|
| D020277 | Polyradiculoneuropathy, Chronic Inflammatory Demyelinating |
| ID | Term |
|---|---|
| D011129 | Polyradiculoneuropathy |
| D020274 | Autoimmune Diseases of the Nervous System |
| D009422 | Nervous System Diseases |
| D003711 | Demyelinating Diseases |
| D011115 | Polyneuropathies |
| D010523 | Peripheral Nervous System Diseases |
| D009468 | Neuromuscular Diseases |
| D001327 | Autoimmune Diseases |
| D007154 | Immune System Diseases |
| D002908 | Chronic Disease |
| D020969 | Disease Attributes |
| D010335 | Pathologic Processes |
| D013568 | Pathological Conditions, Signs and Symptoms |
Not provided
Not provided
| ID | Term |
|---|---|
| D001800 | Blood Specimen Collection |
| ID | Term |
|---|---|
| D013048 | Specimen Handling |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
| D011677 | Punctures |
| D013514 | Surgical Procedures, Operative |
| D008919 | Investigative Techniques |
Not provided
Not provided