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| Name | Class |
|---|---|
| TC Erciyes University | OTHER |
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The purpose of this study was to evaluate the indirect effects of antibiotic combinations. SCAP viability, proliferation and apoptosis of the cells were evaluated. Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated.
The purpose of this study was to evaluate the indirect effects of triple (TAP- ciprofloxacin, metronidazole and minocycline), double (DAP- ciprofloxacin and metronidazole) and modified triple antibiotic pastes (mTAP- ciprofloxacin, metronidazole and cefaclor) on mesenchmal stem cells derived from apical papilla (SCAP). SCAP were isolated from the impacted third molar teeth of the young adults. TAP, DAP and mTAP treatment (14-day medication and 28-day medication) were applied on SCAP and viability, proliferation and apoptosis of the cells were evaluated after four weeks. Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Triple antibiotic paste(TAP) | ciprofloxacin, metronidazole and minocycline |
| |
| Doble antibiotic paste(DAP) | ciprofloxacin and metronidazole |
| |
| Modified triple antibiotic paste(mTAP) | ciprofloxacin, metronidazole and cefaclor |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Differentiation Markers | Drug | Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated. |
|
| Measure | Description | Time Frame |
|---|---|---|
| Measurment of Cell Viability and Apoptosis | To evaluate cell viability and apoptosis MuseCell Analyzer Count and Viability kit (Merck Millipore, Taufkirchen, Germany) was used according to the manufacturer's protocol. | 1 year |
| Measurment of cell differantiation | To determine gene expression levels, total RNA of SCAP was extracted using the Ambion PureLink RNA mini kit (Invitrogen, US) and the Roche cell RNA Kit (Penzberg, Germany) according to the manufacturer's instructions. | 1 year |
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Inclusion Criteria:
Exclusion Criteria:
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Tissue samples were obtained from the apex of the extracted third molar teeth of young adult patients aged between 16-22 .
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| Name | Affiliation | Role |
|---|---|---|
| Seçil Çalışkan | Eskişehir Osmangazi University | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Eskişehir Osmangazi University | Eskişehir | 26040 | Turkey (Türkiye) |
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| ID | Term |
|---|---|
| D000943 | Antigens, Differentiation |
| ID | Term |
|---|---|
| D000954 | Antigens, Surface |
| D000941 | Antigens |
| D001685 | Biological Factors |
| D015415 | Biomarkers |
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Tissue samples were obtained from the apex of the extracted third molar teeth of young adult patients aged between 16-22 (N=7).
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