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The investigators will study nasopharunx microbiota composition, RNA sequences and in vitro cytokines production by peripheral blood cells of COVID-19 patients (both mild-moderate and severe cases) (comparing with convalescent patients and healthy controls).
The investigators will compare study parameters (basal) between mild-moderate and severe COVID-19 patients and with other study groups (i.e. convalescent and healthy controls). Nasopharunx microbiota dysbiosis, in vitro cytokines production profile, and peripheral blood flocytometric results of the four study groups will be compared.
In vitro cytokines production will be studied and compared in Nill (No antigens) and Mitojen (Cocktail of antigens) pre-prepared test tubes.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| 1 | Mild-Moderate COVID-19 |
| |
| 2 | Severe COVID-19 |
| |
| 3 | Convalsent (COVID-19) |
| |
| 4 | Healthy persons |
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Observation | Other | The study parameters will be obtained at the time of recuirement (Basal) |
|
| Measure | Description | Time Frame |
|---|---|---|
| Determination of peripheral blood RNA microarray assay | Four study groups, microarray Analysis . | First day |
| Determination of nasopharunx Microbiota analysis | Four study groups, metagenomic analysis, to determine abundance and decrease of some species. | 1st day |
| Determination of in vitro cytokines production | IL-2, IL-7, IL-10, granulocyte-colony stimulating factor, interferon-p inducible protein 10, monocyte chemo-attracting protein 1, macrophage inflammatory protein 1-α and tumor necrosis factor-α in Nill (no angijen) and Mitojen (Cocktails of antigens)tubes (between 4 study groups) | First day |
| Determination of peripheral blood cytometry | Four study groups, peripheral blood cytometry; CD3, CD4, CD8, and CD45 rates. | First day |
| Relation between study parameters | determining the realtion between microbiotme dysbiosis, in vitro cytokines production and peripheral leymphocytes (CD3,CD4, CD8, and CD45) rates and the severity of COVID-19( if any) | First day parameters |
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Inclusion Criteria:
Exclusion Criteria:
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Adults (18-80 yerars ), both gender
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Mehmet Hursitoglu, MD | Contact | +902129096000 | 20527 | mehmet.hursitoglu@sbu.edu.tr |
| Name | Affiliation | Role |
|---|---|---|
| Mehmet Hursitoglu, MD | Basaksehir Cam and sakura Sehir Hospital | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Bakirkoy Dr.sadi Konuk Training and Research Hospital | Recruiting | Istanbul | 34100 | Turkey (Türkiye) | ||
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| Result | Microbiota and viral infections |
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| ID | Term |
|---|---|
| D000086382 | COVID-19 |
| ID | Term |
|---|---|
| D011024 | Pneumonia, Viral |
| D011014 | Pneumonia |
| D012141 | Respiratory Tract Infections |
| D007239 | Infections |
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| ID | Term |
|---|---|
| D019370 | Observation |
| ID | Term |
|---|---|
| D008722 | Methods |
| D008919 | Investigative Techniques |
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Microbiota and RNA sequencing
| Bakirkoy Dr.sadi Konuk Training and Research Hospital |
| Recruiting |
| Istanbul |
| Turkey (Türkiye) |
| D014777 |
| Virus Diseases |
| D018352 | Coronavirus Infections |
| D003333 | Coronaviridae Infections |
| D030341 | Nidovirales Infections |
| D012327 | RNA Virus Infections |
| D008171 | Lung Diseases |
| D012140 | Respiratory Tract Diseases |