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Since Saline is still the most widely used storage and flushing solution in cardiovascular procedures despite knowing evidence of its influence on the human endothelial cell function the main aim of this study was to assess the effect of DuraGraft©, an intraoperative graft treatment solution, on human saphenous vein segments 8 Remnants from the operation after trimming the bypass length), rat aortic segments and human umbilical vein endothelial cells (HUVECs) in comparison to saline.
Saline is still the most widely used storage and flushing solution in cardiovascular procedures despite knowing evidence of its influence on the human endothelial cell function. Aim of this study was to assess the effect of DuraGraft©, an intraoperative graft treatment solution, on human saphenous vein segments, rat aortic segments and human umbilical vein endothelial cells (HUVECs) in comparison to saline. According to literature saline could have detrimental effects on the endothelium but data shows conflicting evidence. Duragraft a so called endothelial preservation solution should protect the human endothelium. Intent of this observational study was to study the effects of both solutions and compare them.
Within 12 patients undergoing aortocoronary bypass surgery, left overs from saphenous vein graft segments were randomized to DuraGraft© (n=12/6) or saline (n=12/6) solution before intraoperative storage. These segments as well as rat aortic segments underwent assessment of vascular function in a multichamber isometric myograph system in comparison to Krebs-Henseleit solution (KHS), a physiologic organ buffer solution. Additionally, human umbilical vein endothelial cells (HUVECs) were used for cell viability tests.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| A( saline) | The vein piece 1 cm is taken from one patient, then the piece is divided and assigned to saline or the specific solution. |
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| B specific solution | The vein piece 1 cm is taken from one patient, then the piece is divided and assigned to saline or the specific solution. |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| ex vivo organ bath testing, preservation of vein sections without contact to patient | Other | 1 piece of vein is separated during surgical bypass procedure, then randomized and put ex vivo in two solutions: saline and specific solution, then tested in the organ bath |
| Measure | Description | Time Frame |
|---|---|---|
| vessel function organ bath assessment | defines significant contraction or relaxation between the two solutions, a myograph will be used, every change above 5 nM will be seen as significant. | 3 hours |
| Measure | Description | Time Frame |
|---|---|---|
| cell viability testing | shows significant difference between the two solutions, cell number of living cells will be simply counted and compared between the two solutions using a standard microscope and counting chamber | 60 minutes |
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Inclusion Criteria:
Exclusion Criteria:
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patients undergoing bypass surgery exluding emergency cases
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| Name | Affiliation | Role |
|---|---|---|
| Bernhard Winkler, Associate Professor Priv. Doz. DDR. | Klinikum Floridsdorf | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| KH North Vienna | Vienna | 1230 | Austria |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 8267997 | Background | Santoli E, Di Mattia D, Boldorini R, Mingoli A, Tosoni A, Santoli C. University of Wisconsin solution and human saphenous vein graft preservation: preliminary anatomic report. Eur J Cardiothorac Surg. 1993;7(10):548-52. doi: 10.1016/1010-7940(93)90055-g. | |
| 27068248 | Background | Winkler B, Reineke D, Heinisch PP, Schonhoff F, Huber C, Kadner A, Englberger L, Carrel T. Graft preservation solutions in cardiovascular surgery. Interact Cardiovasc Thorac Surg. 2016 Aug;23(2):300-9. doi: 10.1093/icvts/ivw056. Epub 2016 Apr 10. |
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only during publication or manuscript preparation data will be shared but no patients names or data.
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| 39076175 | Derived | Kiss A, Szabo PL, Dostal C, Arnold Z, Geisler D, Crailsheim I, Folkmann S, Grabenwoger M, Podesser BK, Winkler B. Specific Graft Treatment Solution Enhances Vascular Endothelial Function. Rev Cardiovasc Med. 2022 Oct 28;23(11):368. doi: 10.31083/j.rcm2311368. eCollection 2022 Nov. |