The literature has shown that interrupting sitting time with frequent physical activities (e.g., cycling on a stationary bike for 3 minutes every 30 minutes of sitting) is effective for decreasing blood sugar levels. However, the optimal frequency and distribution of sitting interruption remains less clear. The purpose of this study is to compare the effects of two different protocols of sitting interruption (i.e., 3 minutes of cycling every 30 minutes versus 3 minutes of cycling every 15 minutes) on blood sugar levels.

This study will involve two different physical activity conditions and a control condition. Each participant will undergo the three conditions in a random order. The first protocol involves 3 minutes of cycling on a stationary bike at moderate intensity every 30 minutes for 6 hours, totaling 36 minutes of cycling exercise. The second protocol involves 3 minutes of cycling on a stationary bike at moderate intensity every 15 minutes for 3 hours to match the total exercise duration in the first protocol. Participants will remain seated for 3 hours to match to the total time spent in the lab. For the control condition, participants will remain seated. For each condition, participants will be provided with two meals, one before the protocol and another one in the middle of the protocol. The meals will be purchased from the cafeteria on campus based on the participant's own choice as long as carbohydrates account for 55-65% of total calories. Participants will eat identical meals across the three conditions.

Inclusion Criteria:

• Apparently healthy persons
• Sedentary lifestyle

Exclusion Criteria:

• Cardiovascular diseases
• Pulmonary diseases
• Metabolic diseases
• Other contraindications to exercise