In the diagnosis and treatment of patients with sepsis, through routine stool testing and dynamic testing of coagulation function, we found that patients often have stools that are not formed, the proportion of main fecal bacteria is imbalanced, the level of blood bacterial toxins rises, and the abnormal coagulation status indicate the gut microbiome dysbiosis may play an important regulatory role in abnormal blood coagulation in patients with sepsis. Therefore, we propose that the gut microbiome dysbiosis is involved in sepsis-induced coagulopathy. This project intends to prospectively observe the changes in gut microbiome dysbiosis and blood coagulation function in patients with sepsis before and after treatment, and explore whether the changes in gut microbiome dysbiosis promote the development of sepsis through coagulation disorders, provide new research perspectives for diagnosis and treatment for sepsis.

Inclusion Criteria:

• Age ≥18 years and ≤85 years
• Sign the informed consent form
• Expected ICU hospital stay> 24 hours
• △SOFA score ≥2 and confirmed or suspected infection

Exclusion Criteria:

• Pregnant or breastfeeding women
• Estimated survival time <24 hours
• Diagnosis of sepsis time> 24 hours
• The length of ICU stay before diagnosis of sepsis> 7 days
• Sepsis from which the source of infection cannot be determined
• Congenital coagulopathy

Department of Intensive Care Unit, Sun Yat-sen Memorial Hospital, Sun Yat-sen University