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Study of initiating proliferative vitreoretinopathy (PVR)cell subtype (PVR initiating cells (PVR-IC) in RPE cells of rhegmatogenous retinal detachment (RRD) patients; to prove the percentage of PVR-IC decides the risk of serious PVR occurring after surgery; to investigate the safety and efficacy of early local steroids drug intervention in patients with severe postoperative PVR.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| RD control group | Sham Comparator | Conventional Surgery for Retinal Detachment |
|
| RD treatment group1 | Experimental | Conventional Surgery for Retinal Detachment，RPE cell collection, single cell heterogeneity study |
|
| RD treatment group2 | Active Comparator | Conventional Surgery for Retinal Detachment，RPE cell collection, single cell heterogeneity study, postoperative intervention |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| RPE cell collection and single cell heterogeneity study | Diagnostic Test | Study of initiating proliferative vitreoretinopathy (PVR)cell subtype (PVR initiating cells (PVR-IC) in RPE cells of rhegmatogenous retinal detachment (RRD) patients, the percentage of which decides the risk of serious PVR occurring after surgery. |
| Measure | Description | Time Frame |
|---|---|---|
| Retina reattachment rate | Percentage of participants whose fundus achieved the following conditions simultaneously (B-mode ultrasound showed retinal detachment band height was 0mm, OCT showed subretinal fluid height was 0um) | 3 months post operation |
| Number of Severe PVR | Number of participants whose fundus achieved the following conditions simultaneously (B-mode ultrasound showed proliferative band height exceeded 0mm, OCT showed proliferative band exceeded 0um) | 3 months post operation |
| Best Corrected visual Acuity (BCVA） | Patients' best corrected visual acuity assessed by logarithmic visual acuity(0.5-1.0； For example, normal vision was 1.0) | 3 months post operation |
| Intraocular pressure (IOP) | Patients' IOP(mmHg) assessed by noncontact tonometer | 3 months post operation |
| Measure | Description | Time Frame |
|---|---|---|
| Retina reattachment rate | Percentage of participants whose fundus achieved the following conditions simultaneously (B-mode ultrasound showed retinal detachment band height was 0mm, OCT showed subretinal fluid height was 0um) | 12 months post operation |
| Number of Severe PVR |
| Measure | Description | Time Frame |
|---|---|---|
| RD treatment group1and RD treatment group2 | Proportion (%)of PVR-IC subtypes in RPE cells assessed by 10×Genomics single cell sequencing | 1 weeks post operation,3 months post operation,12 months post operation |
| RD treatment group2 |
Inclusion Criteria:
Exclusion Criteria:
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| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Fang Wang, MD,phD | Contact | +86-18917683335 | 18917683335@163.com | |
| Conghui Zhang, MD,phD | Contact | +86-13671886466 | linghui0224@126.com |
| Name | Affiliation | Role |
|---|---|---|
| Fang Wang, MD,phD | Department of ophthalmology, Shanghai Tenth People's Hospital | Principal Investigator |
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| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 31932730 | Background | Moncada R, Barkley D, Wagner F, Chiodin M, Devlin JC, Baron M, Hajdu CH, Simeone DM, Yanai I. Integrating microarray-based spatial transcriptomics and single-cell RNA-seq reveals tissue architecture in pancreatic ductal adenocarcinomas. Nat Biotechnol. 2020 Mar;38(3):333-342. doi: 10.1038/s41587-019-0392-8. Epub 2020 Jan 13. | |
| 31712411 |
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To complete the preliminary report of clinical research.evaluation of the clinical protocol.
2022.12- completing follow-up. Summarizing and analyzing all test data.Comparison of efficacy and summary of adverse reactions.Statistical analysis.Overall outcome evaluation of the clinical protocol.
All access
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|
| Postoperative intervention | Procedure | Early local steroids drug intervention in patients with severe postoperative PVR |
|
| Conventional Surgery for Retinal Detachment | Procedure | Conventional Surgery for Retinal Detachment |
|
Number of participants whose fundus achieved the following conditions simultaneously (B-mode ultrasound showed proliferative band height exceeded 0mm, OCT showed proliferative band exceeded 0um) |
| 12 months post operation |
| Best Corrected visual Acuity (BCVA） | Patients' best corrected visual acuity assessed by logarithmic visual acuity(0.5-1.0； For example, normal vision was 1.0) | 12 months post operation |
| Intraocular pressure (IOP) | Patients' IOP(mmHg) assessed by noncontact tonometer | 12 months post operation |
Patients' IOP (mmHg)assessed by noncontact tonometer; len's opacity(Grade1-4) assessed by PENTACAM; number of participants who occurred subconjunctival chemosis and hemorrhage(area 1/4-1)
| 2 weeks post operation,3 months post operation,12 months post operation |
| Voigt AP, Mulfaul K, Mullin NK, Flamme-Wiese MJ, Giacalone JC, Stone EM, Tucker BA, Scheetz TE, Mullins RF. Single-cell transcriptomics of the human retinal pigment epithelium and choroid in health and macular degeneration. Proc Natl Acad Sci U S A. 2019 Nov 26;116(48):24100-24107. doi: 10.1073/pnas.1914143116. Epub 2019 Nov 11. |
| 31269016 | Background | Hu Y, Wang X, Hu B, Mao Y, Chen Y, Yan L, Yong J, Dong J, Wei Y, Wang W, Wen L, Qiao J, Tang F. Dissecting the transcriptome landscape of the human fetal neural retina and retinal pigment epithelium by single-cell RNA-seq analysis. PLoS Biol. 2019 Jul 3;17(7):e3000365. doi: 10.1371/journal.pbio.3000365. eCollection 2019 Jul. |
| 31230860 | Background | Zhou Y, Liu Z, Welch JD, Gao X, Wang L, Garbutt T, Keepers B, Ma H, Prins JF, Shen W, Liu J, Qian L. Single-Cell Transcriptomic Analyses of Cell Fate Transitions during Human Cardiac Reprogramming. Cell Stem Cell. 2019 Jul 3;25(1):149-164.e9. doi: 10.1016/j.stem.2019.05.020. Epub 2019 Jun 20. |
| 30712875 | Background | Peng YR, Shekhar K, Yan W, Herrmann D, Sappington A, Bryman GS, van Zyl T, Do MTH, Regev A, Sanes JR. Molecular Classification and Comparative Taxonomics of Foveal and Peripheral Cells in Primate Retina. Cell. 2019 Feb 21;176(5):1222-1237.e22. doi: 10.1016/j.cell.2019.01.004. Epub 2019 Jan 31. |
| 29674595 | Background | Filbin MG, Tirosh I, Hovestadt V, Shaw ML, Escalante LE, Mathewson ND, Neftel C, Frank N, Pelton K, Hebert CM, Haberler C, Yizhak K, Gojo J, Egervari K, Mount C, van Galen P, Bonal DM, Nguyen QD, Beck A, Sinai C, Czech T, Dorfer C, Goumnerova L, Lavarino C, Carcaboso AM, Mora J, Mylvaganam R, Luo CC, Peyrl A, Popovic M, Azizi A, Batchelor TT, Frosch MP, Martinez-Lage M, Kieran MW, Bandopadhayay P, Beroukhim R, Fritsch G, Getz G, Rozenblatt-Rosen O, Wucherpfennig KW, Louis DN, Monje M, Slavc I, Ligon KL, Golub TR, Regev A, Bernstein BE, Suva ML. Developmental and oncogenic programs in H3K27M gliomas dissected by single-cell RNA-seq. Science. 2018 Apr 20;360(6386):331-335. doi: 10.1126/science.aao4750. |