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| Name | Class |
|---|---|
| Zuyderland Medisch Centrum | OTHER |
| Maastricht University Medical Center | OTHER |
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Ki-67 is used as a marker for determination of the proliferative activity in solid tumors. The use within hemato-oncological malignancies is limited. This is related to limited technical possibilities of flow cytometry in the past. Meanwhile, flow cytometry in hemato-oncological malignancies has progressed to assessment of 8 colors and makes it possible to add Ki-67 as an additional marker to the 8-color panels. Adding Ki-67 to these panels could lead to improved diagnosis and prediction of therapy response for a number of hemato-oncological malignancies.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Myelodysplastic syndrome (MDS) patients | MDS patients will be divided according to prognostic parameters in sub-cohorts. |
| |
| Acute myeloid Leukemia (AML) patients | AML patients will be divided according to prognostic parameters in sub-cohorts. |
| |
| Myelodysplastic syndrome/neoplasm (MDS/MPN) patients | MDS/MPN patients will be divided according to prognostic parameters in sub-cohorts. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Flow cytometry | Diagnostic Test | Flow cytometric immunophenotyping and determination of proliferative activity by means of Ki-67. |
|
| Measure | Description | Time Frame |
|---|---|---|
| Maturation patterns diagnosis | Maturation patterns based on immunophenotype for red blood cells and several types of immune cells and their respective contributions to diagnosis. Maturation patterns are scored by various methods/combinations to form diagnostic score. A higher diagnostic score will lead to a more likely diagnose for MDS and/or AML. | 5 years |
| Proliferative index diagnosis | Ki-67 proliferative index (within populations and maturation) and its contribution to diagnosis. A lower Ki-67 proliferative index will lead to a more likely diagnose for MDS and/or AML. | 5 years |
| Proliferative index prognosis | Ki-67 as prognostic parameter. A lower Ki-67 proliferative index will (hypothetically) lead to worse prognosis for MDS and AML in terms of: transfusion dependence (expressed in amount of transfusions in 2 months), chemotherapy response (expressed as total remission, normalization of blood values, possibly also normalization of cytogenetics in bone marrow cells), overall survival (expressed in months after diagnosis), Risk scores. Higher risk scores are correlated with worse prognosis. | 5 years |
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Inclusion Criteria:
Exclusion Criteria:
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Patients in which a hemato-oncological malignancy is found and are at least 18 years old. These patients can be male and female.
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Mathie Leers, Dr. | Contact | +31 45 5767503 | mat.leers@zuyderland.nl | |
| Bart de Wit, Dr. | Contact | +31 43 3874694 | b.de.wit@mumc.nl |
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| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 30176186 | Background | Nies KPH, Kraaijvanger R, Lindelauf KHK, Drent RJMR, Rutten RMJ, Ramaekers FCS, Leers MPG. Determination of the proliferative fractions in differentiating hematopoietic cell lineages of normal bone marrow. Cytometry A. 2018 Nov;93(11):1097-1105. doi: 10.1002/cyto.a.23564. Epub 2018 Sep 3. |
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| ID | Term |
|---|---|
| D009190 | Myelodysplastic Syndromes |
| D015470 | Leukemia, Myeloid, Acute |
| D000754 | Anemia, Refractory, with Excess of Blasts |
| ID | Term |
|---|---|
| D001855 | Bone Marrow Diseases |
| D006402 | Hematologic Diseases |
| D006425 | Hemic and Lymphatic Diseases |
| D007951 | Leukemia, Myeloid |
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| ID | Term |
|---|---|
| D005434 | Flow Cytometry |
| ID | Term |
|---|---|
| D002469 | Cell Separation |
| D003584 | Cytological Techniques |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
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| D007938 | Leukemia |
| D009370 | Neoplasms by Histologic Type |
| D009369 | Neoplasms |
| D000753 | Anemia, Refractory |
| D000740 | Anemia |
| D003933 | Diagnosis |
| D003592 | Cytophotometry |
| D005470 | Fluorometry |
| D008163 | Luminescent Measurements |
| D010783 | Photometry |
| D002623 | Chemistry Techniques, Analytical |
| D008919 | Investigative Techniques |