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| Name | Class |
|---|---|
| Institute of Oncology Research (IOR) | UNKNOWN |
| Istituto Cantonale di Patologia | OTHER |
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The study includes 2 sub-projects.
Sub-project 1: The aim is to evaluate the expression of receptors and activating proteases mediating SARS-CoV-2 entry and spreading in the local population of Ticino.
Sub-project 2: The aim is to investigate the association between the HSD3B1 gene variations and outcome of COVID-19 in the local population of Ticino.
Sub-project 1:
This is an observational retrospective study that aims to evaluate the expression of receptors and activating proteases mediating SARS-CoV-2 entry and spreading in the local population of Ticino. The level of expression of these factors will be compared between infected and non-infected subjects. The sub-project will examine RNA samples from nasopharyngeal swabs taken from subjects tested for COVID-19.
Sub-project 2:
This is a haplotype- and diplotype-based case-control retrospective study that aims to investigate the association between the HSD3B1 gene variations and outcome of COVID-19 in the local population of Ticino. The gene status of HSD3B1 will be defined and compared between two groups of patients: the patients who experienced a severe SARS-CoV-2 infection (severe pulmonary insufficiency, ICU admission, eventually death) and the group with a mild-moderate COVID-19 disease without pulmonary insufficiency and oxygen need.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Patients tested for COVID-19 | The nasopharyngeal swabs taken from patients tested for Covid-19 who resulted infected and non-infected will be analyzed to evaluate the expression of receptors and activating proteases mediating SARS-CoV-2. |
| |
| Patients with mild-moderate and severe SARS-CoV-2 infection | Formalin-fixed paraffin will be analysed to determine the association between polymorphism of the HSD3B1 gene and outcomes in COVID-19 affected patients |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Expression of receptors and activating proteases | Genetic | Level of expression of the mRNA of the genes encoding ACE2, TMPRSS2, TMPRSS4 and AR along with reference genes (beta-actin and EPCAM) in nasopharyngeal swabs taken from patients tested for COVID-19 |
| Measure | Description | Time Frame |
|---|---|---|
| Expression of receptors and activating proteases | Determination of the level of expression of the mRNA genes | 1 year |
| Prevalencen of olymorphisms of the HSD3B1 | Determination of the prevalence of polymorphisms of the HSD3B1 patients with severe and mild-moderate SARS-CoV-2 infection | 1 year |
| Association of polymorphisms of the HSD3B1 | Evaluation of the association of polymorphisms of the HSD3B1 with clinico-pathological parameters | 1 year |
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Sub-project 1
Inclusion Criteria:
Exclusion Criteria:
- Documented refusal.
Sub-project 2
Inclusion Criteria:
Exclusion Criteria:
- Documented refusal.
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Subjects tested for COVID-19
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| Name | Affiliation | Role |
|---|---|---|
| Ricardo Pereira Mestre, MD | Oncology Institute of Southern Switzerland (IOSI) | Study Chair |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Oncology Institute of Southern Switzerland (IOSI) | Bellinzona | 6500 | Switzerland | |||
| Clinica Luganese Moncucco |
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| ID | Term |
|---|---|
| D000086382 | COVID-19 |
| ID | Term |
|---|---|
| D011024 | Pneumonia, Viral |
| D011014 | Pneumonia |
| D012141 | Respiratory Tract Infections |
| D007239 | Infections |
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Sub-project 1: nasopharyngeal swabs
Sub-project 2: tissue samples
| Polymorphism of the HSD3B1 | Genetic | Formalin-fixed paraffin embedded tissue blocks will be amplified using polymerase chain reaction (PCR) based experiments followed by direct sequencing (Sanger sequencing) |
|
| Lugano |
| 6900 |
| Switzerland |
| D014777 |
| Virus Diseases |
| D018352 | Coronavirus Infections |
| D003333 | Coronaviridae Infections |
| D030341 | Nidovirales Infections |
| D012327 | RNA Virus Infections |
| D008171 | Lung Diseases |
| D012140 | Respiratory Tract Diseases |