The purpose of this study is to investigate the effect of prolonged overnight fasting in the hepatic glycogen stores

Inclusion Criteria:

• Participants are able to provide signed and dated written informed consent prior to any study specific procedures
• Caucasian (people will be excluded when having a ≥50% racial African/Asian background)
• Participants should have suitable veins for cannulation or repeated venipuncture
• Women are post-menopausal (defined as at least 1 year post cessation of menses)
• Men and women aged ≥ 45 and ≤ 75 years at the start of the study
• Body mass index (BMI) 18.5 - 25 kg/m2
• Stable dietary habits (no weight loss or gain >3kg in the past 3 months)
• Sedentary lifestyle (not more than 3 hours of vigorous sports per week)

Exclusion Criteria:

• Type 2 Diabetes Mellitus
• Active diseases (cardiovascular, diabetes, liver, kidney, cancer or other)
• Any contraindication for MRI scanning
• Alcohol consumption of >2 servings per day
• Regular smoking
• No use of medication interfering with investigated study parameters (as determined by responsible physician)
• Participants who do not want to be informed about unexpected medical findings, or do not wish that their physician