In this project, we aim at implementing a te-ASL sequence and to compare it to two reference ASL sequences with a fixed PLD on healthy individuals. On the one hand, the te-ASL sequence will be compared against a 3D pCASL with a PLD of 1800 ms, as the current reference according the Consensus of the ISMRM perfusion study group and the European consortium for ASL in dementia. On the other, as the implemented te-ASL sequence will have a 2D readout, it will also be compared to a 2D pCASL ASL sequence with the same PLD, in order to be able to disentangle differences due to the dynamic PLD scheme from those arising from the different read-out schemes.

Inclusion Criteria:

• To sign the study informed consent form approved by the corresponding authorities.
• Healthy men and women (older than 18 years old).

Exclusion Criteria:

• Inability or unwillingness to give written informed consent or communicate with the study staff or illiteracy.
• Any contraindication to perform brain MRI procedure (e.g. pacemaker, MRI-incompatible aneurysm clips).

Thirty (N=30) healthy individuals older than 18 years old