Effects of Silica and Asbestos Fibres on the Efferocytosis Capacities of Macrophages

Effects of Silica and Asbestos Fibres on the Efferocytosis Capacities of Macrophages

Evaluation of the efferocytosis capacities of blood Monocyte Derived Macrophages (MDM) from patients with a history of asbestosis or silica exposure and comparison of these capacities with those of MDM from healthy donors.

Patients with a proven history or current exposure will have 4 EDTA tubes drawn, approximately 24mL of fresh whole blood for efferocytosis assessment and :

• 2 x 6mL serum tubes for the assessment of Antinuclear Antibody (ANA) positivity, serum high-mobility group box 1 (HMGB1) and osteopontin for patients exposed to asbestos
• 1 x 6mL serum tube for serum HMGB1 and osteopontin for patients exposed to silica

Inclusion Criteria:

• Patient older than 18 Yo
• Addressed to the department of occupational disease
• With a history of asbestosis or silica exposure evaluated by a dedicated questionnaire and a throughout examination of the history of occupations, according to standard procedures for the follow-up of at risk workers.
• Persons who received an oral and written information on the protocol and signed the informed consent form

Exclusion Criteria:

• Pregnant or breastfeeding women
• Subject legally protected (under judicial protection, guardianship), persons deprived of liberty