Determination of the pubertal growth spurt in Egyptian female patients using gingival crevicular fluid alkaline phosphatase

assessment of the pubertal growth spurt in growing Egyptian female patients by using a simple, quick and easy method, through taking a gingival crevicular fluid alkaline phosphatase sample and measuring its concentration. Comparing the concentration of GCF ALP to the standardized middle phalanax of the middle finger MP3 we can predict if it can be an accurate and reliable method as skeletal growth indicator.

Inclusion Criteria:

1. Gender: only female subjects will be included.
2. Age between 7-16 years old
3. Intermediate or late mixed dentition and permanent dentition
4. Good general health with absence of any nutritional problems or bony disease
5. No usage of any anti-inflammatories or antibiotics in the month preceding the study
6. Fully erupted upper and lower central incisors for sample collection
7. Good gingival and periodontal health as indicated by Probing depth (PD) values that should not exceed 3 mm for the whole dentition.
8. Willingness to participate in the study and sign the informed consent form

Exclusion Criteria:

1. Male adolescent subjects
2. Periodontal and /or gingival disease
3. Systemic and/or bone disease

3. Growth abnormality

self representation of gender identity

Egyptian female patients with age range from 7 to 16 years old