The objective of this study is to investigate the effect of the infection control measures based on the active screening of carbapenem-resistant Klebsiella pneumoniae and whole-genome based tracking and surveillance though the hospital.

Inclusion Criteria:

1. The patient was admitted to the ICU of neurosurgery in the west hospital of huashan hospital affiliated to fudan university from September 2019 to September 2021, or transferred from the ICU of another hospital
2. The patient is more than 18 years old and less than 80 years old, regardless of gender.
3. Informed consent of the patient;

Exclusion Criteria:

1. Patients participating in other clinical trials at the same time
2. Pregnant

4. People who will affect the outcome of the study: whose specimens could not be received or not enough for testing