The purpose of this study is to determine the effect of measured and induced negative feelings on glucose, tumor necrosis factor alpha, and cortisol levels in healthy participants and participants with type 2 diabetes mellitus.

Depression and Diabetes are two pathologies that are frequently comorbid. Stress and negative thoughts are part of both. The biological correlates of psychological and emotional life are not yet fully clarified, so in this study we wonder about the association between tumor necrosis factor alpha, cortisol, and peripheral glucose levels with depression stress, measured negative thoughts, and induced guilt.This is a randomized intervention study. At baseline, participants complete the Beck Depression Inventory, the Automatic Negative Thoughts Scale (ATQ-N), and the Perceived Stress Scale (PSS-14). The intervention consists of a written task about situations that involved feelings of guilt or neutral situations. Saliva and dextrostix samples are taken before the 20-minute written task, at the end, dextrostix and saliva samples are performed again, finally a blood sample is taken.

Inclusion Criteria:

• Written, informed consent.
• 30 to 65 years of age.
• Current diagnosis of Diabetes mellitus type 2 without diabetic foot.
• Healthy volunteers.
• Ability to read and write.

Exclusion Criteria:

• Other current or past chronic disease diagnoses.
• Current use of antidepressants or other psychiatric medications.
• Steroid medication.
• Consumption of tobacco or alcohol in the last 24 hours.
• Previous diagnosis of autoimmune diseases