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| ID | Type | Description | Link |
|---|---|---|---|
| H19-02684 | Other Identifier | UBC Clinical Research Ethics Board |
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This study will determine whether acute ingestion of a high fat "Bulletproof Coffee" will lead to changes in plasma triglycerides, immune cell function, as well as cognitive function when compared to a black coffee.
In a randomized crossover design, participants will consume a single bulletproof coffee or a single black coffee separated by ~7 days. Each visit commences in the morning after an overnight fast of 10-12 hours, with no exercise the day prior, no coffee consumption the morning of testing, and completion of a food log for dietary duplication prior to visit 2. A fasting blood draw is obtained and cognitive function tests administered, along with questionnaires to assess gastrointestinal distress, arousal, and hunger/fullness before coffee consumption. Following this, one of the coffee beverages is consumed (randomized to each visit). Cognitive testing and questionnaires are completed at 60 minutes after finishing the coffee, another blood draw at 90 minutes, and then cognitive testing, questionnaires and a final blood draw 180 minutes after consuming the coffee. This procedure is repeated during their second experimental visit, in which they consume the remaining coffee based on their randomization.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Bulletproof Coffee | Experimental | A black coffee (bullet proof coffee keurig pod) with 29 grams of added fat (1 tablespoon bullet proof brain octane medium chain triglyceride oil, and 1 tablespoon bullet proof grass fed ghee) blended for 20 seconds with butter scent that is added to the lid. |
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| Black Coffee | Placebo Comparator | Black coffee that is blended for 20 seconds with butter scent added to the top of the lid. |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| High-fat coffee | Dietary Supplement | A high fat coffee that consists of 29 grams of added fat from grass fed ghee, and MCT oil. |
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| Measure | Description | Time Frame |
|---|---|---|
| Plasma triglycerides | Circulating triglycerides measured in mmol/L | Change over 3 hours following beverage consumption |
| Measure | Description | Time Frame |
|---|---|---|
| Cognitive function | Measures of cognitive function including Stroop test, digit substitution, and reaction time measured via computerized test battery. | Change over 3 hours following beverage consumption |
| Immune Cell Activation |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Garett S Jackson, B.H.K | Contact | 1-250-807-9122 | garett11@mail.ubc.ca | |
| Jonathan P Little, PhD | Contact | 1-250-878-6893 | jonathan.little@ubc.ca |
| Name | Affiliation | Role |
|---|---|---|
| Jonathan P Little, PhD | University of British Columbia- Okanagan | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| University of British Columbia, Okanagan. | Recruiting | Kelowna | British Columbia | V1V 1V7 | Canada |
Data used for publication will be shared in a raw data format. This will include participant ID, and all participant characteristics with no personally identifying information.
The study protocol and informed consent form will be available immediately upon study publication. The statistical analytical plan will be outlined in detail in the manuscript, however if more details are required, these will be provided. All information will be provided for 36 months following publication of results.
IPD will be provided to credentialed research institutions/university labs. This data may be used for meta-analyses as well as direction for pilot work and future studies. Upon query, it will be determined whether the requesting party is eligible to view the data, both to maintain anonymity of the participants, and to ensure adequate, ethical and responsible use of IPD.
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Randomized double-blind crossover design.
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| Black coffee | Dietary Supplement | A regular black coffee with no added caloric content. |
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Assessment of activation markers on monocytes and neutrophils measured as median fluorescence intensity by flow cytometry.
| Change over 3 hours following beverage consumption |
| ID | Term |
|---|---|
| D007964 | Leukocytosis |
| ID | Term |
|---|---|
| D007960 | Leukocyte Disorders |
| D006402 | Hematologic Diseases |
| D006425 | Hemic and Lymphatic Diseases |
| D010335 | Pathologic Processes |
| D013568 | Pathological Conditions, Signs and Symptoms |
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