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| Name | Class |
|---|---|
| Zhongda Hospital | OTHER |
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EVTRNA is to analyze the differentiated expression pattern of circular RNA (circRNA), long non-coding RNA (lncRNA) and micro-RNA (miRNA) by next-generation sequencing in acute ischemic stroke patients before and/or after endovascular treatment. The candidate circRNA/lncRNA/miRNA will be verified as the biomarker and regulator for progression and prognosis of acute ischemic stroke with endovascular treatment. Further, the candidate non-coding RNA will be used to evaluate the effect of endovascular treatment on both peripheral and central immune after stroke.
Noncoding RNAs have been highlighted to be involved in the pathological process of ischemic stroke (IS). The purpose of this protocol will investigate the differentiated expression pattern of circular RNA (circRNA), long non-coding RNA (lncRNA) and micro-RNA (miRNA) by next-generation sequencing in acute ischemic stroke patients before and/or after endovascular treatment. The candidate circRNA/lncRNA/miRNA will be verified as the biomarker and regulator for progression and prognosis of acute ischemic stroke with endovascular treatment. Further, the candidate non-coding RNA will be used to evaluate the effect of endovascular treatment on both peripheral and central immune after stroke. Distinctive expression patterns of circRNA/miRNA/lncRNA will be identified by the next-generation sequencing and individual quantitative real time polymerase chain reaction (qRT-PCR). A predictive model will be established using logistic regression. The panel of these altered ncRNAs may be associated with the immune status after acute IS and could serve as a regulator for progression and prognosis of acute ischemic stroke with endovascular treatment.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| AIS before EVT group | This group includes patients with acute ischemic stroke (AIS) before endovascular treatment (EVT) |
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| AIS after EVT group | This group includes patients with acute ischemic stroke (AIS) after endovascular treatment (EVT) |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Sequencing of circRNA/lncRNA/miRNA | Genetic | Next generation sequencing and quantitative real-time polymerase chain reaction of circular RNA (circRNA), long non-coding RNA (lncRNA) and micro-RNA (miRNA) |
| Measure | Description | Time Frame |
|---|---|---|
| Differential expression pattern of circRNA/lncRNA/miRNA in acute ischemic stroke patients before and after endovascular treatment | Differential expression pattern of circular RNA (circRNA), long non-coding RNA (lncRNA) and micro-RNA (miRNA) will be compared in acute ischemic stroke patients before and after endovascular treatment, thus candidate circRNA/lncRNA/miRNA will be verified as biomarkers and regulators for progression and prognosis of acute ischemic stroke with endovascular treatment. | 90 days |
| Measure | Description | Time Frame |
|---|---|---|
| Prognostic value of circRNA/miRNA/lncRNA in acute ischemic stroke with endovascular treatment | The prognosis of AIS patients will be analyzed between differential expressed candidate circRNA/lncRNA/miRNA after they have endovascular treatment. | 90 days |
| Correlation of circRNA/lncRNA/miRNA and inflammatory factors in acute ischemic stroke with endovascular treatment |
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Inclusion Criteria:
Exclusion Criteria:
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There will be 5 AIS patients, whose data before and after endovascular treatment will be applied for sequencing the differentiated expression pattern and constraction of diagnosis and predictive models of circRNA/lncRNA/miRNA from the circulating blood. There will be 300 AIS patients, whose data will be applied for validation of such diagnosis and predictive models and investigated the effect of non-coding RNA on immune status after endovascular treatment.
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Junshan Zhou, M.D | Contact | +8602587726218 | zhjsh333@126.com | |
| Qiwen Deng, M.D | Contact | +8602587726218 | qiw_deng@163.com |
| Name | Affiliation | Role |
|---|---|---|
| Junshan Zhou, M.D | Nanjing First Hospital, Nanjing Medical University | Study Chair |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Nanjing First Hospital, Nanjing Medical University | Recruiting | Nanjing | Jiangsu | 210006 | China |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 29997237 | Background | Deng QW, Li S, Wang H, Sun HL, Zuo L, Gu ZT, Lu G, Sun CZ, Zhang HQ, Yan FL. Differential long noncoding RNA expressions in peripheral blood mononuclear cells for detection of acute ischemic stroke. Clin Sci (Lond). 2018 Jul 31;132(14):1597-1614. doi: 10.1042/CS20180411. Print 2018 Jul 31. |
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| ID | Type | URL | Comment |
|---|---|---|---|
| Clinical Study Report | View IPD |
All information will be available to all researchers
All information will be available to all researchers when related investigation has been accepted publicly, and will be available for 5 years.
All information will be available to all researchers when related investigation has been accepted publicly.
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| ID | Term |
|---|---|
| D020521 | Stroke |
| D000083242 | Ischemic Stroke |
| ID | Term |
|---|---|
| D002561 | Cerebrovascular Disorders |
| D001927 | Brain Diseases |
| D002493 | Central Nervous System Diseases |
| D009422 | Nervous System Diseases |
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| ID | Term |
|---|---|
| D060888 | Real-Time Polymerase Chain Reaction |
| ID | Term |
|---|---|
| D016133 | Polymerase Chain Reaction |
| D021141 | Nucleic Acid Amplification Techniques |
| D005821 | Genetic Techniques |
| D008919 | Investigative Techniques |
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An 10 ml peripheral venous blood will be collected from the participants before and after endovascular treatment
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Correlation of circRNA/lncRNA/miRNA and inflammatory factors in acute ischemic stroke before and after endovascular treatment will be explored. |
| 90 days |
| Correlation of circRNA/lncRNA/miRNA and stroke-associated infection | Correlation of circRNA/lncRNA/miRNA and stroke-associated infection will be explored in acute ischemic stroke with endovascular treatment. | 90 days |
| Dynamic changes of circRNA/lncRNA/miRNA during the follow-up period | Dynamic changes of circRNA/lncRNA/miRNA will be explored after endovascular therapy during the follow-up period | 90 days |
| D014652 | Vascular Diseases |
| D002318 | Cardiovascular Diseases |