Osteoarthritis (OA) is a highly progressive and debilitating joint disease. Recently, it was demonstrated the existence of an important link between OA inflammation and progression of structural changes. Therapy with intra-articular injection of stromal vascular fraction (SVF) holds great promises and its efficacy could be further augmented with the addition of biological adjuvants. In the present project, we aim at demonstrating that human platelet lysate (HPL) increases the intrinsic beneficial properties of SVF on cartilage regeneration and joint environment. Moreover, we want to verify if SVF-conditioned medium (CM) obtained from SVF primed with HPL yields comparable or superior outcomes than whole SVF.

Inclusion Criteria:

OA patients

• Subscription of informed consent
• BMI < 30
• age between 55-75 years included
• Kelgrenn-Lawrence equal or superior to grade III
• presence of synovitis
• patients undergoing knee replacement
• suspension of NSAIDs from one week before the surgical procedure according to the standard clinical practice Healthy subjects from aesthetic plastic surgery
• Subscription of informed consent
• BMI < 30
• age between 18-50 years included
• subjects undergoing liposuction and/or abdominoplasty

Exclusion Criteria:

• HCV, HIV, HBV, TPHA infection
• Pregnancy (auto declaration)

Healthy subjects undergoing aesthetic plastic surgery or OA patients undergoing prosthetic surgery