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| ID | Type | Description | Link |
|---|---|---|---|
| 15/10/19 | Other Identifier | Local Ethics Commission | |
| 02785 | Other Identifier | UMG study center |
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The aim of this study is to investigate whether smoking is associated with changes in salivary composition and/or predisposition to erosion.
Healthy volunteers are observationally wearing an intraoral device with both bovine tooth specimens (enamel and dentin) and resin specimens twice for two hours each. Afterwards, specimens are eroded extraorally and calcium release into the acid is measured.
Total protein concentration and protein composition of the salivary pellicles on the resin samples are measured. Additionally, salivary parameters (unstimulated and stimulated saliva flow rate, pH, buffer capacity, total protein content and protein composition as well as concentration of inorganic calcium, phosphate, and fluoride) are measured.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Smokers | Volunteers smoking at least 10 cigarettes per day |
| |
| Non-smokers | Non-smoking volunteers |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Use of fluoridated toothpaste | Other | Use of fluoridated toothpaste |
| |
| Measure | Description | Time Frame |
|---|---|---|
| Calcium release from bovine enamel and dentin specimens by extraoral erosion (nmol/Square Millimeter). | Immediately after the intraoral device has been worn once for two hours. |
| Measure | Description | Time Frame |
|---|---|---|
| Determination of unstimulated and stimulated saliva flow rate (mL/min). | Saliva samples are collected for 5 min each on three visits. | |
| Determination of saliva pH (pH). | Saliva samples are collected for 5 min each on three visits. |
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Inclusion Criteria:
Exclusion Criteria:
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Exploratory study
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| Name | Affiliation | Role |
|---|---|---|
| Annette Wiegand, Prof. Dr. med. dent. | Dept. of Prev. Dentistry, Periodontology and Cariology, University Medical Center Göttingen, Germany | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| University Medical Center Göttingen, Dept. of Preventive Dentistry, Periodontology and Cariology | Göttingen | Lower Saxony | 37075 | Germany |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 24603346 | Background | Carpenter G, Cotroneo E, Moazzez R, Rojas-Serrano M, Donaldson N, Austin R, Zaidel L, Bartlett D, Proctor G. Composition of enamel pellicle from dental erosion patients. Caries Res. 2014;48(5):361-7. doi: 10.1159/000356973. Epub 2014 Mar 6. | |
| 24993266 | Background | Moazzez R, Bartlett D. Intrinsic causes of erosion. Monogr Oral Sci. 2014;25:180-96. doi: 10.1159/000360369. Epub 2014 Jun 26. |
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| ID | Term |
|---|---|
| D014077 | Tooth Erosion |
| ID | Term |
|---|---|
| D017001 | Tooth Demineralization |
| D014076 | Tooth Diseases |
| D009057 | Stomatognathic Diseases |
| D057085 | Tooth Wear |
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Saliva samples are obtained and retained for 10 years.
| Wearing of an intraoral device with bovine tooth samples |
| Other |
Wearing of an intraoral device with bovine tooth samples |
|
| Wearing of an intraoral device with resin samples | Other | Wearing of an intraoral device with resin samples |
|
| Determination of salivary buffer capacity (pH). | Saliva samples are collected for 5 min each on three visits. |
| Determination of total protein content (mg/L) and protein composition (qualitatively) in saliva. | Saliva samples are collected for 5 min each on three visits. |
| Determination of inorganic calcium (mmol/L), phosphate (mmol/L) and fluoride (µmol/L) in saliva. | Saliva samples are collected for 5 min each on three visits. |
| Determination of total protein concentration (ng/Square Millimeter) and protein composition (qualitatively) within the salivary pellicles. | Immediately after the intraoral device with resin samples has been worn once for two hours. |
| 18607835 | Background | Wiegand A, Bliggenstorfer S, Magalhaes AC, Sener B, Attin T. Impact of the in situ formed salivary pellicle on enamel and dentine erosion induced by different acids. Acta Odontol Scand. 2008 Aug;66(4):225-30. doi: 10.1080/00016350802183401. |
| 18523383 | Background | Wiegand A, Meier W, Sutter E, Magalhaes AC, Becker K, Roos M, Attin T. Protective effect of different tetrafluorides on erosion of pellicle-free and pellicle-covered enamel and dentine. Caries Res. 2008;42(4):247-54. doi: 10.1159/000135669. Epub 2008 Jun 4. |
| 23774653 | Background | Zwier N, Huysmans MC, Jager DH, Ruben J, Bronkhorst EM, Truin GJ. Saliva parameters and erosive wear in adolescents. Caries Res. 2013;47(6):548-52. doi: 10.1159/000350361. Epub 2013 Jun 15. |
| 27022647 | Background | Kanzow P, Wegehaupt FJ, Attin T, Wiegand A. Etiology and pathogenesis of dental erosion. Quintessence Int. 2016 Apr;47(4):275-8. doi: 10.3290/j.qi.a35625. |
| 31163440 | Background | Wiegand A, Rosemann A, Hoch M, Barke S, Dakna M, Kanzow P. Erosion-Protective Capacity of the Salivary Pellicle of Female and Male Subjects Is Not Different. Caries Res. 2019;53(6):636-642. doi: 10.1159/000500046. Epub 2019 Jun 4. |
| 41577165 | Result | Kanzow P, Lenz C, Schlarmann F, Barke S, Rohland B, Schmidt A, Gorshkova A, Neuenroth L, Wiegand A. Saliva and salivary pellicle composition and proteomic profile in smokers vs. non-smokers and its effect on dental erosion. J Dent. 2026 Mar;166:106517. doi: 10.1016/j.jdent.2026.106517. Epub 2026 Jan 21. |