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| Name | Class |
|---|---|
| Université du Québec a Montréal | OTHER |
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This study aims to obtain the lacrimal fingerprint for frequent pathologies of the ocular surface and establish a normative base for each of them.
This is an exploratory study. The investigators aim to:
During regular clinics at the cornea service of the ophthalmology department of the CHUM (Centre Hospitalier de l'Université de Montréal), the investigators aim to recruit a few hundred patients with various pathologies of the ocular surface, notably different forms of dry eye syndrome, infectious keratitis/conjunctivitis, mucous membrane pemphigoid and allergic conjunctivitis, and collect a sample of their tears via Schirmer strip. A case report form will be completed for each patient, noting known ocular diagnoses and active topical ophthalmic medication. Schirmer strips will be sent in sterile tubes to UQAM (Université du Québec à Montréal)'s department of chemistry for analysis by mass spectrometry.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Control group | Healthy, normal ocular surface |
| |
| Dry eye syndrome | Patients suffering from either:
|
| |
| Allergic conjunctivitis | Patients suffering from allergic conjunctivitis |
| |
| Mucous membrane pemphigoid | Patients suffering from mucous membrane pemphigoid |
| |
| Infectious keratoconjunctivitis | Patients suffering from keratitis and/or conjunctivitis of various etiology:
|
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Tear sample collection via Schirmer strip and subsequent analysis by mass spectrometry | Diagnostic Test | During regular consultations at the ophthalmology department of the CHUM, eligible patients will undergo a standard 5 minute Schirmer tear test. The Schirmer strips will serve as tear samples and will be sent to UQAM's Department of chemistry for mass spectrometry analysis. |
| Measure | Description | Time Frame |
|---|---|---|
| To obtain the metabolomic and proteomic fingerprint of the studied ocular surface pathologies | Raw data will be analysed with MarkerView software by Sciex to identify specific over- or under-expressed markers in these ocular pathologies. | 5 years |
| Measure | Description | Time Frame |
|---|---|---|
| To objectify changes in metabolomic and proteomic profile associated with topical treatments taken by participants in this study | Raw data will be analysed with the same software to identify specific changes in proteomic and/or metabolomic expression associated with the topical treatments taken by participants in this study. | 5 years |
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Inclusion Criteria:
Dry eye syndrome; Infectious keratitis and/or conjunctivitis; Mucous membrane pemphigoid; Allergic conjunctivitis.
Exclusion Criteria:
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Patients consulting at the cornea service of the ophthalmology department of the CHUM.
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Marie-Claude Robert, MD, M.Sc | Contact | 514-890-8278 | marie-claude.robert.2@umontreal.ca | |
| Marie-Catherine Tessier | Contact | 514-890-8000 | 11550 | marie-catherine.tessier.chum@ssss.gouv.qc.ca |
| Name | Affiliation | Role |
|---|---|---|
| Marie-Claude Robert, MD, M.Sc | Centre hospitalier de l'Université de Montréal (CHUM) | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Université du Québec à Montréal (UQAM) - Department of Chemistry | Active, not recruiting | Montreal | Quebec | H2X 2J6 | Canada |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 15911533 | Background | Karring H, Thogersen IB, Klintworth GK, Moller-Pedersen T, Enghild JJ. A dataset of human cornea proteins identified by Peptide mass fingerprinting and tandem mass spectrometry. Mol Cell Proteomics. 2005 Sep;4(9):1406-8. doi: 10.1074/mcp.D500003-MCP200. Epub 2005 May 23. | |
| 24921002 | Background | Elsobky S, Crane AM, Margolis M, Carreon TA, Bhattacharya SK. Review of application of mass spectrometry for analyses of anterior eye proteome. World J Biol Chem. 2014 May 26;5(2):106-14. doi: 10.4331/wjbc.v5.i2.106. |
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The investigators do not plan to share individual participant data.
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| Centre Hospitalier de l'Université de Montréal (CHUM) | Recruiting | Montreal | Quebec | H2X 3E4 | Canada |
|
| 22634083 | Background | Zhou L, Zhao SZ, Koh SK, Chen L, Vaz C, Tanavde V, Li XR, Beuerman RW. In-depth analysis of the human tear proteome. J Proteomics. 2012 Jul 16;75(13):3877-85. doi: 10.1016/j.jprot.2012.04.053. Epub 2012 May 23. |
| 23769845 | Background | Karnati R, Laurie DE, Laurie GW. Lacritin and the tear proteome as natural replacement therapy for dry eye. Exp Eye Res. 2013 Dec;117:39-52. doi: 10.1016/j.exer.2013.05.020. Epub 2013 Jun 12. |
| 16901338 | Background | de Souza GA, Godoy LM, Mann M. Identification of 491 proteins in the tear fluid proteome reveals a large number of proteases and protease inhibitors. Genome Biol. 2006;7(8):R72. doi: 10.1186/gb-2006-7-8-R72. Epub 2006 Aug 10. |
| 27413414 | Background | Hagan S, Martin E, Enriquez-de-Salamanca A. Tear fluid biomarkers in ocular and systemic disease: potential use for predictive, preventive and personalised medicine. EPMA J. 2016 Jul 13;7(1):15. doi: 10.1186/s13167-016-0065-3. eCollection 2016. |
| 28736338 | Background | Willcox MDP, Argueso P, Georgiev GA, Holopainen JM, Laurie GW, Millar TJ, Papas EB, Rolland JP, Schmidt TA, Stahl U, Suarez T, Subbaraman LN, Ucakhan OO, Jones L. TFOS DEWS II Tear Film Report. Ocul Surf. 2017 Jul;15(3):366-403. doi: 10.1016/j.jtos.2017.03.006. Epub 2017 Jul 20. |
| 23272196 | Background | Aluru SV, Agarwal S, Srinivasan B, Iyer GK, Rajappa SM, Tatu U, Padmanabhan P, Subramanian N, Narayanasamy A. Lacrimal proline rich 4 (LPRR4) protein in the tear fluid is a potential biomarker of dry eye syndrome. PLoS One. 2012;7(12):e51979. doi: 10.1371/journal.pone.0051979. Epub 2012 Dec 18. |
| 20508732 | Background | Enriquez-de-Salamanca A, Castellanos E, Stern ME, Fernandez I, Carreno E, Garcia-Vazquez C, Herreras JM, Calonge M. Tear cytokine and chemokine analysis and clinical correlations in evaporative-type dry eye disease. Mol Vis. 2010 May 19;16:862-73. |
| 18334958 | Background | Green-Church KB, Nichols KK, Kleinholz NM, Zhang L, Nichols JJ. Investigation of the human tear film proteome using multiple proteomic approaches. Mol Vis. 2008 Mar 7;14:456-70. |
| ID | Term |
|---|---|
| D015352 | Dry Eye Syndromes |
| D007639 | Keratoconjunctivitis, Infectious |
| D010390 | Pemphigoid, Benign Mucous Membrane |
| D003233 | Conjunctivitis, Allergic |
| ID | Term |
|---|---|
| D007766 | Lacrimal Apparatus Diseases |
| D005128 | Eye Diseases |
| D015818 | Eye Infections, Bacterial |
| D001424 | Bacterial Infections |
| D001423 | Bacterial Infections and Mycoses |
| D007239 | Infections |
| D015817 | Eye Infections |
| D007637 | Keratoconjunctivitis |
| D003231 | Conjunctivitis |
| D003229 | Conjunctival Diseases |
| D007634 | Keratitis |
| D003316 | Corneal Diseases |
| D000820 | Animal Diseases |
| D012872 | Skin Diseases, Vesiculobullous |
| D012871 | Skin Diseases |
| D017437 | Skin and Connective Tissue Diseases |
| D006969 | Hypersensitivity, Immediate |
| D006967 | Hypersensitivity |
| D007154 | Immune System Diseases |
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