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High salt intake is a major risk factor for cardiovascular events, including stroke, heart disease and kidney failure. Pizza is a popular salty food. In this exploratory pilot study, the effects of a pizza meal challenge on immunometabolism in healthy subjects will be tested.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Pizza meal challenge | Experimental | Vegetarian pizza containing 10 grams of salt |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Pizza meal challenge | Other | Vegetarian pizza containing 10 grams of salt |
|
| Measure | Description | Time Frame |
|---|---|---|
| Oxygen consumption rate of immune cells | OCR (pmol oxygen/min/1000 cells) measured by Seahorse | 3 hours after the pizza compared to baseline |
| Measure | Description | Time Frame |
|---|---|---|
| Oxygen consumption rate of immune cells | OCR (pmol oxygen/min/1000 cells) measured by Seahorse | 3 and 8 hours after the pizza adjusted for plasma sodium concentration compared to baseline |
| Plasma sodium |
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Inclusion Criteria:
- Healthy men and women
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Dominik N. Müller, PhD | Charite University, Berlin, Germany | Principal Investigator |
| Anja Mähler, PhD | Charite University, Berlin, Germany | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Experimental & Clinical Research Center | Berlin | 13125 | Germany |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 33906377 | Result | Geisberger S, Bartolomaeus H, Neubert P, Willebrand R, Zasada C, Bartolomaeus T, McParland V, Swinnen D, Geuzens A, Maifeld A, Krampert L, Vogl M, Mahler A, Wilck N, Marko L, Tilic E, Forslund SK, Binger KJ, Stegbauer J, Dechend R, Kleinewietfeld M, Jantsch J, Kempa S, Muller DN. Salt Transiently Inhibits Mitochondrial Energetics in Mononuclear Phagocytes. Circulation. 2021 Jul 13;144(2):144-158. doi: 10.1161/CIRCULATIONAHA.120.052788. Epub 2021 Apr 28. |
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Individual participant data that underlie the results of reported articles (text, tables, figures, supplemental data) will be shared after deidentification.
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Beginning 9 months and ending 36 months following article publication.
Researchers who provide a methodologically sound proposal.
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Plasma sodium concentration (mmol/l)
| 3 and 8 hours after the pizza compared to baseline |
| Mitochondrial membrane potential of immune cells | Mitochondrial membrane potential (Mean Fluorescence Intensity of TMRE) measured by flow cytometry | 3 and 8 hours after the pizza compared to baseline |
| Extracellular acidification rate of immune cells | ECAR (mpH/min/1000 cells) measured by Seahorse | 3 and 8 hours after the pizza compared to baseline |
| Interleukin 17 release after monocyte and T cell co-culture | IL-17 concentration (pg/ml) measured by ELISA | Cell isolation at baseline and 3 hours after the pizza followed by 72 hours of co-culture |
| Interferon-gamma release after monocyte and T cell co-culture | INF-g concentration (pg/ml) measured by ELISA | Cell isolation at baseline and 3 hours after the pizza followed by 72 hours of co-culture |
| Adenosine triphophate production of immune cells | Percent change of luminescence (%) measured by Luminescence Assay | 3 and 8 hours after the pizza compared to baseline |