The Impact of Body Mass Index on the Endometrial Microbiome in IVF Treatments. Tampere Infertility Microbiome Project (TIMP).

A Protocol for a Prospective Study to Compare the Endometrial Microbiome in Patients With Body Mass Index Over 30 kg/m² Versus Under 25 kg/m².

This study project is an observational trial involving 90 patients suffering from male type infertility. The patients are divided in two groups (45 patients per group) based on their body mass index (BMI).

This study (Tampere Infertility Microbiome Project, TIMP) aims to clarify the differences in the microbiome variety between obese and normal-weight patients. The first group will consist of patients with the body mass index (BMI) over 30 kg/m², while the second group will include patients with a healthy weight BMI under 25. The samples will be collected at the time of embryo transfer and analyzed in a laboratory specialing in next-generation sequencing (NGS) techniques.

Inclusion Criteria:

• Patients with male infertility scheduled for IVF treatment and fresh embryo transfer
• Primary or secondary male-type infertility
• BMI over 30 kg/m² or under 25 kg/m²
• Patient's willingness to participate

Exclusion Criteria:

• Patients with endometriosis or adenomyosis
• Use of antibiotics during IVF treatment or oocyte retrieval
• Technical diffuculties in embryo transfer or no embryos available for transfer