Regenerative endodontic procedures have been used to successfully treat human mature permanent teeth with necrotic pulps and apical periodontitis. Many researchers have begun to apply regenerative endodontic procedures to mature teeth in adult patients. Several clinical case reports have shown complete resolution of signs and symptoms of pulp necrosis in mature teeth, even those with large periapical lesions, as well as signs of pulp canal obliteration. thevaim of this study is To assess the regenerative potential of mature permanent teeth with necrotic pulps with platelet-rich fibrin (PRF) and blood clot using radiographic and clinical methods.

Inclusion Criteria:

• Patients with mature teeth.
• Patients with periapical periodontitis.
• Maxillary anterior teeth only will be involved.
• Patients should be free from any systemic disease that may affect normal healing and predictable outcome.
• Patients who will agree to the consent and will commit to follow up period.

Exclusion Criteria:

• Patients with immature roots.
• Patients with any systemic disease that may affect normal healing.
• Patients with swelling.
• Pregnant females.
• Patients who could/would not participate in a 1 year follow up.
• Patients with fistula.
• Patients with old age.
• Teeth with periodontal involvement.
• Teeth with vertical root fractures.
• Unrestorable teeth.