Electronic Hookah and Endothelial Cell Function

The Effects of Electronic Hookah on Endothelial Cell Function: The Role of Nicotine

Electronic nicotine delivery systems (ENDS) are a rapidly growing global epidemic among adolescents and young adults. Unlike other ENDS such as e-cigarettes, e-hookahs are used through traditional water-pipes, allowing the vapor-containing nicotine, propylene glycol, glycerin, and flavorings-to pass through a water-filled basin, potentially altering the vapor, before it is inhaled through the user's mouth. Contributing to e-hookahs popularity is the belief that the flavored smoke is detoxified as it passes through the water-filled basin, rendering e-hookah a safer tobacco alternative. However, an e-hookahs deliver flavored nicotine by creating a vapor of fine particles and volatile organic compounds that could induce vascular toxicity. While e-hookah vaping acutely reduces endothelial function, the specific role of nicotine and the mechanisms by which it may impairs endothelial function remain understudied. The objective of this project is to investigate the specific role of nicotine in mediating the acute effects of e-hookah vaping on endothelial dysfunction.

Inclusion Criteria:

• 21-39 years old hookah smokers: smoked hookah >12x in last 12 months
• 21-39 years old e-cigarette users: vaped >12x in last 12 months
• no history of illicit drugs
• no evidence of cardiopulmonary disease by history/ physical
• no diabetes: fasting blood glucose <100 mg/dl
• BP<140/90mmHg
• resting HR<100 bpm
• BMI<30kg•m2
• no prescription medication

Exclusion Criteria:

• exhaled CO>10 ppm (smoking non-abstinence)
• positive pregnancy test
• psychiatric illness