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Purpose: The purpose of this study was to evaluate the possible application of metabolomics to identify follicular fluid changes in cancer patients undergoing fertility preservation. Although metabolomics have been applied already in cancer studies, this is the first application on follicular fluid of cancer patients.
Methods: The investigators selected for the study 10 patients with breast cancer and lymphoma who resorted to oocyte cryopreservation to preserve fertility and 10 healthy women undergoing in vitro fertilization treatments. Follicular fluid was collected at the time of oocytes retrieval. Metabolomic analysis of follicular fluids was performed by 1H NMR spectroscopy in combination with multivariate analysis to interpret the spectral data. Univariate statistical analysis was applied to find correlations between patients' features and metabolites identified by NMR.
The purpose of this study was to evaluate the possible application of metabolomics to identify follicular fluid changes in cancer patients undergoing fertility preservation. Although metabolomics have been applied already in cancer studies, this is the first application on follicular fluid of cancer patients.
Methods: The investigators selected for the study 10 patients with breast cancer and lymphoma who resorted to oocyte cryopreservation to preserve fertility and 10 healthy women undergoing in vitro fertilization treatments. Follicular fluid was collected at the time of oocytes retrieval. Metabolomic analysis of follicular fluids was performed by 1H NMR spectroscopy in combination with multivariate analysis to interpret the spectral data. Univariate statistical analysis was applied to find correlations between patients' features and metabolites identified by NMR.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| healthy subjects | 10 subjects |
| |
| oncological patients | 10 subjects |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| oocytes retrieval | Procedure | The oocytes retrieval was performed after 34-36 hours from hCG administration . The collection of cumulus-oocyte complexes and FFs were performed via transvaginal ultrasound-guided aspiration with a needle of 18 gauge of diameter. The three major diameter follicles were identified by intraoperative ultrasound and FF of each follicle was collected in its own test tube. |
| Measure | Description | Time Frame |
|---|---|---|
| follicular fluid metabolites of patients with neoplasia | identification by NMR spectroscopy of the metabolites present in the follicular fluid of woman | NMR spectroscopy was performed immediately after oocyte retrieval |
| Measure | Description | Time Frame |
|---|---|---|
| to define the existence of cancer-specific metabolic profiles | to study by NMR spectroscopy of the existence of cancer-specific metabolic profiles present in the follicular fluid of woman resorting to fertility preservation | NMR spectroscopy was performed immediately after oocyte retrieval |
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Inclusion Criteria:
Exclusion Criteria:
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oncological patients with pathological diagnosis resorting fertility preservation
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| Name | Affiliation | Role |
|---|---|---|
| Maria Antonietta MACM Castiglione Morelli, Professor | University of Basilicata studies | Principal Investigator |
| Assunta Iuliano, MD | San Carlo Public Hospital, Potenza | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Angela Ostuni | Potenza | 85100 | Italy |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 30069850 | Background | Castiglione Morelli MA, Iuliano A, Schettini SCA, Petruzzi D, Ferri A, Colucci P, Viggiani L, Cuviello F, Ostuni A. NMR metabolomics study of follicular fluid in women with cancer resorting to fertility preservation. J Assist Reprod Genet. 2018 Nov;35(11):2063-2070. doi: 10.1007/s10815-018-1281-7. Epub 2018 Aug 1. |
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| Type | Date | Date Unknown |
|---|---|---|
| Release | Apr 5, 2021 | |
| Reset | Apr 29, 2021 | |
| Release | May 23, 2024 | |
| Reset | Sep 20, 2024 |
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| Release Date | Unrelease Date | Unrelease Date Unknown | Reset Date | MCP Release Number |
|---|---|---|---|---|
| Apr 5, 2021 | Apr 29, 2021 | |||
| May 23, 2024 |
| ID | Term |
|---|---|
| D054315 | Oocyte Retrieval |
| ID | Term |
|---|---|
| D027724 | Reproductive Techniques, Assisted |
| D012099 | Reproductive Techniques |
| D013812 | Therapeutics |
| D020858 | Tissue and Organ Harvesting |
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follicular fluids
|
| Sep 20, 2024 |
| D014180 | Transplantation |
| D013514 | Surgical Procedures, Operative |
| D008919 | Investigative Techniques |