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Uterine natural killer (uNK) cells have been implicated to play a role in female reproductive performance. Serval studies showed an increased uNK cell density in the endometrium of women with reproductive failure. In this study, we wish to investigate the density and clustering of four different immune cells.
Implantation is a process whereby the embryo attaches itself to the endometrium. In humans, the endometrium has a specific period of uterine receptivity, which occurs 5-7 days after ovulation and remains receptive for ~4 days (cycle days 20-24), when embryo implantation starts. Implantation failure refers to the failure of the embryo to reach a stage when intrauterine gestational sac is recognized by ultrasonography. It occurs when the embryo is abnormal or when endometrial function is impaired.
Uterine natural killer (uNK) cells have been implicated to play a role in female reproductive performance. They comprise 70-80% of the total leukocyte population in the endometrium during implantation and early pregnancy. Several studies showed an increased uNK cell density in the endometrium of women with reproductive failure. To better understand the contribution of various immune factors to endometrial function and future pregnancy, it is proposed to examine the major immune cells in the same specimen to measure the relative magnitude of the cell density changes and the degree of clustering between two immune cell types.
The aim of this study is to investigate the endometrial leucocytes profile around the time of embryo implantation.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| women who are undergoing frozen embryo transfer | endometrial biopsy will be done one cycle before the frozen embryo transfer |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| endometrial biopsy | Procedure | . Endometrial samples are obtained using a biopsy catheter as follows: the Pipette is inserted through the cervical os and advanced gently until resistance is felt. The inner piton of the device is then withdrawn to create suction, and the endometrial sample is obtained by moving the pipette up and down approximately 2-3 cm within the uterine cavity. The device is rotated 360° to ensure adequate coverage of the area. |
| Measure | Description | Time Frame |
|---|---|---|
| immune cell counting | The counting method will be performed according to an agreed and published standardized protocol by a multicenter working group designed to reduce variance of results between centres. Ten images of 40X magnification fields will be analyzed for each sample. The first field to be captured should be selected at random, ensuring that it contained the luminal epithelial border. Subsequent fields should be obtained by moving one field to the left or right of the original field, keeping the luminal epithelial border in view. | up to 2 months |
| Quantification of endometrial immune cells spatial distribution | In each 200 times under the microscope vision, based on the X and Y position of each single cell which InForm system could provide, the relative spatial distribution of each individual endometrial immune cell will be considered as a bivariate point pattern | up to 2 months |
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Inclusion Criteria:
Exclusion Criteria:
Endometrial polyp or/ and Large fibroid with a diameter >4 cm
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Endometrial biopsies will be collected for all the participants
Women undergoing frozen embryo transfer.
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Wing Ching Cheung, PhD | Contact | 85235051764 | cosycheung@cuhk.edu.hk | |
| Xiaoyan Chen, PhD | Contact | 85235051764 | chenxiaoyan@cuhk.edu.hk |
| Name | Affiliation | Role |
|---|---|---|
| Xiaoyan Chen, PhD | Chinese University of Hong Kong | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| The Chinese University of Hong Kong | Recruiting | Hong Kong | Hong Kong |
Please contact the principle investigator if there is any enquiry
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endometrial samples will be stored
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