Breast milk is an optimal source of nutrients for newborns as well as a potential source of bacteria for the intestinal microbiota. However, there are limited number of studies evaluating the relationship between maternal nutrition, breast milk and neonatal intestinal microbiota.

The study was conducted on a total of 20 healthy women following at Obstetrics and Gynecology Department of Gülhane Education and Research Hospital in Ankara Health Sciences University. Sociodemographic characteristics, anthropometric measurements, breastfeeding characteristics were evaluated in the third trimester of the women who underwent routine prenatal care. Breast milk samples were obtained at 16.day postpartum during lactation.

Inclusion Criteria:

• Singleton pregnancy
• Age 19-35 years
• At weeks of 30-36 gestation
• No history of acute or chronic diseases
• Term delivery

Exclusion Criteria:

• Alcohol use
• Smoking
• Gestational diabetes
• Type 1 and 2 diabetes
• Preeclampsia
• Polycystic ovary
• Syndrome
• Thyroid and parathyroid disease
• Metabolic bone or kidney disease
• Abnormal liver function
• Multiple pregnancy
• Premature and postmature labor
• Having chemotherapy
• Taking drugs such as antibiotics and probiotics

20 healthy women who were at 16. day postpartum