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| Name | Class |
|---|---|
| Centre National de la Recherche Scientifique, France | OTHER |
| Sys2Diag, Mixt laboratory CNRS/Alcediag, Montpellier | UNKNOWN |
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The study aims at the identification and the quantification of urinary branched-chain amino acids (BCAAs) by mass spectometry in healthy subjects and patients with insulin resistance and correlating urinary BCAAs with HOMA index.
Blood and urine levels of BCAAs will be correlated with HOMA index to assess and quantify insulin resistance.
Identification and quantification of urine BCAAs by an innovative method based on synthetic biology will also be performed.
Correlations between mass spectometry and the innovative detection method of BCAAs will be searched.
Blood and urine samples will be collected in 330 persons to assess BCAAs levels and correlate them with HOMA index.
Persons included will be:
110 persons with BMI>27 and HOMA >4 with no diabetes 110 persons with BMI>27 and HOMA < 4 with no diabetes 110 persons with BMI < 27 and HOMA <4 with no diabetes, including at least 50 men and 50 women, 30 aged 18-35, 30 aged 36-55, 30 aged 56-75.
Urine BCAAs will be measured by both mass spectrometry and the innovative method based on synthetic biology.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| HEALTHY SUBJECTS | Experimental | SUBJECTS WITH BMI<27 and HOMA<4 |
|
| OVERWEIGHT NON INSULINRESISTANT PATIENTS | Experimental | SUBJECTS WITH BMI>27 and HOMA<4 |
|
| OVERWEIGHT INSULINRESISTANT PATIENTS | Experimental | SUBJECTS WITH BMI>27 and HOMA>4 |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| assessment of biomarkers of insulin resistance | Diagnostic Test | blood and urine sample collection for assays of BCAAs |
|
| Measure | Description | Time Frame |
|---|---|---|
| Correlation between urinary levels of BCAAs and HOMA index | Identification of urinary BCAAs as markers of insulin resistance | through study completion, an average of two years and a half |
| Measure | Description | Time Frame |
|---|---|---|
| Correlation between blood and urine BCAAs levels | Validation of urinary BCAAs as biomarkers of insulin resistance | through study completion, an average of two years and a half |
| Correlation between urine BCAAs levels measured by enzymatic method (artificial vesicles) and classic method |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| EMILIE BARDE | UH Montpellier | Study Director |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| UH Montpellier | Montpellier | 34295 | France |
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| ID | Term |
|---|---|
| D007333 | Insulin Resistance |
| ID | Term |
|---|---|
| D006946 | Hyperinsulinism |
| D044882 | Glucose Metabolism Disorders |
| D008659 | Metabolic Diseases |
| D009750 | Nutritional and Metabolic Diseases |
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Cohort of subjects with/without overweight and with/without insulin resistance
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Measured by enzymatic method (artificial vesicles) and classic method (standard LC-MS/MS) |
| through study completion, an average of two years and a half |
| Correlation between urine BCAAs levels measured by enzymatic method (artificial vesicles) and HOMA index | Measured by enzymatic method (artificial vesicles) and HOMA index | through study completion, an average of two years and a half |