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| Name | Class |
|---|---|
| Institut de Génétique Moléculaire de Montpellier | OTHER |
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The authors hypothesize that there is a correlation between the percentage of CD4+ T cells expressing CD32a and/or X and the quantity of DNA found in peripheral blood mononuclear cells in patients infected with HIV-1. Also, that there is a correlation between expression of CD32a and/or X and proviral load.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| HIV-A infected patients |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Bioclinical evaluation | Other | 50-100ml blood extracted for flow cytometry and qPCR |
|
| Measure | Description | Time Frame |
|---|---|---|
| Percentage of CD4+ T cells expressing CD32 alone | % | Day 0 |
| Percentage of CD4+ T cells expressing X alone | % | Day 0 |
| Percentage of CD4+ T cells expressing both CD32 and X | % | Day 0 |
| Quantification of proviral load | Quantitative PCR; number of copies of HIV-1 DNA per million peripheral blood mononuclear cells | Day 0 |
| Measure | Description | Time Frame |
|---|---|---|
| Duration of infection prior to treatment | Months | Day 0 |
| Duration of treatment | Months | Day 0 |
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Inclusion Criteria:
Exclusion Criteria:
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Patients with aviremic HIV-1 undergoing antiretroviral treatment for at least 2 years. Patients will be divided into 3 groups according to pre-therapeutic CD4+ T cell levels:
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| Name | Affiliation | Role |
|---|---|---|
| Pierre Corbeau, MD | CHU Nimes | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| CHU de Nimes | Nîmes | 30029 | France |
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| Year treatment commenced | Year | Day 0 |
| Pre-therapeutic CD4 + T cell count | Number of CD4+ T cells/ microL blood | Day 0 |
| Pre-therapeutic viremia | Number of copies of HIV-1 RNA/ml plasma | Day 0 |
| Change in CD4+ T cells over previous 2 years | Number of CD4+ T cells/microL blood lost per year | Day 0 |
| Change in CD4+ T cells prior to treatment | Number of CD4+ T cells/microL blood lost per year | Day 0 |
| Change in CD4+ T cells during treatment | Number of CD4+ T cells/microL blood lost per year | Day 0 |
| Viremia at inclusion into the study | Number of copies of HIV-1 RNA/ml plasma | Day 0 |
| Viremia during the 2 previous years | Number of copies of HIV-1 RNA/ml plasma | Day 0 |
| Residual immune activation at inclusion into the study | CD4/CD8 ratio | Day 0 |
| Residual immune activation during the previous 2 years | CD4/CD8 ratio | Day 0 |
| Nature of current treatment | Family of molecule | Day 0 |
| Co-infection with hepatitis C virus | Yes/No | Day 0 |
| Co-infection with hepatitis B virus | Yes/No | Day 0 |
| Co-infection with Cytomegalovirus | Yes/No | Day 0 |
| Co-infection with Epstein-Barr virus | Yes/No | Day 0 |
| Testing for intact proviral DNA | Number of copies/million cells | Day 0 |
| Circulating viral RNA sequence | RNA sequence | Day 0 |