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The comprehensive analysis of health records, TCM constitution, biomarker, and whole-genome sequencing among dry eye syndrome, healthy control, Sjögren's syndrome and other inflammation disease in Taiwan: an integrated analysis between Taiwan Biobank and Sjögren's syndrome Database
Method: This study wants to apply the dataset of health records, physical examination indicators, blood biomarker, urine biomarker, biochemical biomarker, gene sequence, and metabolome from Taiwan Biobank Database to explore the difference among dry eye syndrome (DES), healthy control (HC), and other inflammation disease (ID). In addition, we want to investigate the difference among the demographics, personal health behavior, living environment, dietary status, family history, Traditional Chinese medicine (TCM) body constitution, hematology test, serum biochemical test, virus test, and urine test. We also want to use the whole-genome genotyping, whole-genome sequencing, DNA methylation, HLA typing, and metabolome to investigate the difference among the genetic variant, gene copy number, single nucleotide variation, chromosomal recombination, and very low-level gene expression differences. It could find the pathogenesis-associated candidate genes and sites, and could apply to clinical diagnosis and drug development after verification and confirmation of efficacy. Integrated the Sjögren's syndrome (SJS) database, we could explore the comprehensive analysis among DES, HC, SJS, and ID. It would provide the evidence-base medicine in TCM in the future.
Expected Results:
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Dry eye syndrome | Dry eye syndrome patients were extracted from Taiwan Biobank. |
| |
| Health subjects | Health subjects were extracted from Taiwan Biobank. |
| |
| Sjögren's syndrome | Sjogren's syndrome patients were screened in the rheumatology outpatient departments and Center for Traditional Medicine at Taipei Veterans General Hospital, and were enrolled with the inclusion criteria: (1) aged between 20 and 75 years; (2) fulfilled the 2002 American-European Consensus Criteria for SS (AECG) |
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Whole-genome genotyping | Diagnostic Test | Using Whole-genome genotyping from Taiwan Biobank among dry eye syndrome, healthy control, Sjögren's syndrome and other inflammation disease. |
| Measure | Description | Time Frame |
|---|---|---|
| Immunity-related gene expression | To evaluate the immunity-related gene expression for Dry eye syndrome, Sjögren's syndrome and healthy control. DNA exome combine the GATK (Genome Analysis Toolkit) is a tool for detecting the ratio of immunity-related gene expression. | Five year |
| TCM pattern | To evaluate the difference of TCM pattern between Dry eye syndrome, Sjögren's syndrome and healthy control. TCM pattern is a tool for detecting the constitution. | Five year |
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Sjögren's syndrome
Inclusion Criteria:
Dry eye syndrome
Inclusion Criteria:
Inflammation disease
Inclusion Criteria:
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Health Subjects
Inclusion Criteria:
Exclusion Criteria:
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Ching-Mao Chang, M.D., Ph.D. | Contact | 88628757453 | 333 | magicbjp@gmail.com |
| Name | Affiliation | Role |
|---|---|---|
| Ching-Mao Chang, M.D., Ph.D. | Taipei Veterans General Hospital, Taiwan | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Ching-Mao Chang | Taipei | Taiwan (r.o.c.) | 112 | Taiwan |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 40724666 | Derived | Huang PY, Chen CH, Chiu YF, Lin HC, Chang CM. Dietary Habits, TCM Constitutions, and Obesity: Investigating the Protective Effects of Vegetarian Dietary Patterns in Taiwan. Healthcare (Basel). 2025 Jul 8;13(14):1641. doi: 10.3390/healthcare13141641. |
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The DNA of Sjögren's syndrome patients, dry eye syndrome patients, other inflammation disease, and health subjects will take to do the DNA Exome sequencing.
The saliva of Sjögren's syndrome patients, other inflammation disease, and health subjects will take to do the oral microbiome.
The protein of Sjögren's syndrome patients, other inflammation disease, and health subjects will take to do the proteomics.
The RNA of Sjögren's syndrome patients, dry eye syndrome patients, other inflammation disease, and health subjects will store to do the RNA sequencing.
The plasma of Sjögren's syndrome patients, dry eye syndrome patients, other inflammation disease, and health subjects will store to do the cytokine detection in the future.
|
| other inflammation disease | Other inflammation disease were extracted from Taiwan Biobank. |
|
| Whole-genome sequencing | Diagnostic Test | Using whole-genome sequencing from Taiwan Biobank among dry eye syndrome, healthy control, Sjögren's syndrome and other inflammation disease to investigate the difference among the genetic variant, gene copy number, single nucleotide variation, chromosomal recombination, and very low-level gene expression differences. |
|
| TCM pattern | Diagnostic Test | We want to use TCM pattern to investigate the difference among dry eye syndrome, healthy control, Sjögren's syndrome and other inflammation disease. |
|
| DNA Exome sequencing | Diagnostic Test | The DNA of dry eye syndrome, healthy control, Sjögren's syndrome and other inflammation disease will take to do the DNA Exome sequencing. |
|
| Physical examination indicators, blood, urine and biochemical function test | Diagnostic Test | To investigate the difference of Physical examination indicators, blood, urine and biochemical function test among dry eye syndrome, healthy control, Sjögren's syndrome and other inflammation disease. |
|
| Demographics, personal health behavior, living environment, dietary status, family history | Diagnostic Test | To investigate the difference among the demographics, personal health behavior, living environment, dietary status, family history of among dry eye syndrome, healthy control, Sjögren's syndrome and other inflammation disease. |
|
| HLA typing | Diagnostic Test | Using HLA typing from Taiwan Biobank among dry eye syndrome, healthy control, Sjögren's syndrome and other inflammation disease to investigate the difference among the genetic variant, gene copy number, single nucleotide variation, chromosomal recombination, and very low-level gene expression differences. |
|
| Full-length 16S Amplicon Sequencing | Diagnostic Test | Using Full-length 16S Amplicon Sequencing from Taiwan Biobank among dry eye syndrome, healthy control, Sjögren's syndrome and other inflammation disease to investigate the difference among the genetic variant, gene copy number, single nucleotide variation, chromosomal recombination, and very low-level gene expression differences. |
|
| Epigenomic | Diagnostic Test | Using Epigenomic from Taiwan Biobank among dry eye syndrome, healthy control, Sjögren's syndrome and other inflammation disease to investigate the difference among the genetic variant, gene copy number, single nucleotide variation, chromosomal recombination, and very low-level gene expression differences. |
|
| Metabolomics | Diagnostic Test | Using Metabolomics from Taiwan Biobank among dry eye syndrome, healthy control, Sjögren's syndrome and other inflammation disease to investigate the difference among the genetic variant, gene copy number, single nucleotide variation, chromosomal recombination, and very low-level gene expression differences. |
|
| Environmental Toxic | Diagnostic Test | Using Environmental Toxic from Taiwan Biobank among dry eye syndrome, healthy control, Sjögren's syndrome and other inflammation disease to investigate the difference among the genetic variant, gene copy number, single nucleotide variation, chromosomal recombination, and very low-level gene expression differences. |
|
| Medical Image | Diagnostic Test | Using Medical Image from Taiwan Biobank among dry eye syndrome, healthy control, Sjögren's syndrome and other inflammation disease to investigate the difference among the genetic variant, gene copy number, single nucleotide variation, chromosomal recombination, and very low-level gene expression differences. |
|
| ID | Term |
|---|---|
| D015352 | Dry Eye Syndromes |
| D012859 | Sjogren's Syndrome |
| D007249 | Inflammation |
| D014985 | Xerophthalmia |
| ID | Term |
|---|---|
| D007766 | Lacrimal Apparatus Diseases |
| D005128 | Eye Diseases |
| D001172 | Arthritis, Rheumatoid |
| D001168 | Arthritis |
| D007592 | Joint Diseases |
| D009140 | Musculoskeletal Diseases |
| D012216 | Rheumatic Diseases |
| D014987 | Xerostomia |
| D012466 | Salivary Gland Diseases |
| D009059 | Mouth Diseases |
| D009057 | Stomatognathic Diseases |
| D003240 | Connective Tissue Diseases |
| D017437 | Skin and Connective Tissue Diseases |
| D001327 | Autoimmune Diseases |
| D007154 | Immune System Diseases |
| D010335 | Pathologic Processes |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D003229 | Conjunctival Diseases |
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| ID | Term |
|---|---|
| D001800 | Blood Specimen Collection |
| D014554 | Urination |
| D009752 | Nutritional Status |
| D008487 | Medical History Taking |
| D006650 | Histocompatibility Testing |
| ID | Term |
|---|---|
| D013048 | Specimen Handling |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
| D011677 | Punctures |
| D013514 | Surgical Procedures, Operative |
| D008919 | Investigative Techniques |
| D014553 | Urinary Tract Physiological Phenomena |
| D012101 | Reproductive and Urinary Physiological Phenomena |
| D009747 | Nutritional Physiological Phenomena |
| D000066888 | Diet, Food, and Nutrition |
| D010829 | Physiological Phenomena |
| D006304 | Health Status |
| D003710 | Demography |
| D011154 | Population Characteristics |
| D007159 | Immunologic Tests |
| D007158 | Immunologic Techniques |
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