Validation of a NICS Method Using the Culture Medium

Validation of a Noninvasive Chromosome Screening (NICS) Method Using the Culture Medium

This study aims to validate a non-invasive method of chromosomal screening (NICS), based on the Multiple Annealing and Looping Based Amplification Cycles- Next-generation Sequencing (MALBAC-NGS) technology, in order to determine the chromosomal endowment of a blastocyst from the DNA of the embryonic culture medium.

The chromosomal status of the embryo from an embryo biopsy of trophoectoderm will be established (usual clinical practice), the chromosomal endowment from the DNA of the embryonic culture medium will be determined, and the results using the NICS and the conventional invasive method (Preimplantation Genetic Testing for Aneuploidy [PGT-A]) will be compared.

Inclusion Criteria:

• Treatment by oocyte donation or own oocyte nd preimplantation genetic screening of aneuploidies (PGT-A).
• Embryonic cryopreservation.
• Informed consent signature

Exclusion Criteria:

• Embryo transfer without embryo freezing
• Embryonic block.
• Bad embryo quality.