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The project examines over several days how the distribution pattern of dietary protein stimulate and affect the protein turnover of important proteins in the aging perspective, such as skeletal muscle proteins.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| MINCED-SKEWED | Placebo Comparator | Minced meat administered with a skewed protein distribution pattern |
|
| MINCED-EVEN | Experimental | Minced meat administered with an even protein distribution pattern |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Distribution pattern of dietary proteins (minced meat) | Other | Study effect of an even or skewed distribution pattern of dietary protein |
|
| Measure | Description | Time Frame |
|---|---|---|
| Muscle protein synthesis | Myofibrillar FSR measured by deuterated water technique, by which deuterium labeled alanine incorporation into myofibrillar muscle protein synthesis can be measured. | 3 days |
| Net protein balance | Whole body net protein balance measured during 10 hours acute study. Measured by the application of stable amino acid labeled phenylalanine and tyrosine tracers, by which the protein synthesis, phenylalanine oxidation and protein breakdown can be calculated. | 10 hours |
| Measure | Description | Time Frame |
|---|---|---|
| D5-phenylalanine incorporation | D5-phenylalanine incorporation into myofibrillar proteins. Dietary meat proteins are intrinsically labeled with D5-phenylalanine | 3 days |
| Muscle protein synthesis |
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Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Institute of Sports Medicine Copenhagen | Copenhagen | DK-2400 | Denmark |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 37086618 | Derived | Agergaard J, Justesen TEH, Jespersen SE, Tagmose Thomsen T, Holm L, van Hall G. Even or skewed dietary protein distribution is reflected in the whole-body protein net-balance in healthy older adults: A randomized controlled trial. Clin Nutr. 2023 Jun;42(6):899-908. doi: 10.1016/j.clnu.2023.04.004. Epub 2023 Apr 8. |
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| ID | Term |
|---|---|
| D055948 | Sarcopenia |
| ID | Term |
|---|---|
| D009133 | Muscular Atrophy |
| D020879 | Neuromuscular Manifestations |
| D009461 | Neurologic Manifestations |
| D009422 | Nervous System Diseases |
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Randomized controlled
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Myofibrillar protein synthesis (%/hour) measured during 10 hours of the acute study. Calculated from the difference in D8-phenylalanine incorporation from the muscle biopsies taken at 2 hours and 12 hours into the stable amino acid tracer infusion.
| 10 hours |
| Protein synthesis | Whole body protein synthesis (%/hour) measured during acute study, by the use of stabel amino acid tracer technique. | 10 hours |
| Protein breakdown | Whole body protein breakdown (%/hour) measured during acute study, by the use of stabel amino acid tracer technique. | 10 hours |
| Phenylalanine oxidation | Phenylalanine oxidation measured during acute study | 10 hours |
| D5-phenylalanine appearance | Plasma D5-phenylalanine enrichment after intake of D5-phenylalanine intrinsically labelled meat proteins | 12 hours |
| Signaling pathways in skeletal muscle | Intracellular signaling pathways related to protein turnover. Measured by Western blotting technique from the muscle tissue biopsies taken at 2 hours and 12 hours into the acute study. | 10 hours |
| Plasma insulin | Plasma insulin concentration during acute study | 12 hours |
| D001284 | Atrophy |
| D020763 | Pathological Conditions, Anatomical |
| D013568 | Pathological Conditions, Signs and Symptoms |
| D012816 | Signs and Symptoms |