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Due to the long half-life (~36 hr) of clenbuterol, detection methods such as dried blood spots (DBS) are a potentially suitable method to easily and non-invasively detect doping misuse of this compound for several days after ingestion. If, and how long, the compound can be detected by DBS has not yet been investigated but is of interest due to its potential in doping-control. The aim is to evaluate whether abuse of clenbuterol can be detected at relevant concentration levels in samples obtained using DBS and to assess the physiological response to clenbuterol in skeletal muscle..
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Clenbuterol | Experimental | Subjects ingest 80 micrograms of clenbuterol tablets |
|
| Placebo | Placebo Comparator | Subjects ingest placebo tablets |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Clenbuterol Oral Product | Drug | Subjects ingest 4x20 microgram clenbuterol tablets |
| |
| Measure | Description | Time Frame |
|---|---|---|
| Blood clenbuterol concentration | Concentration of clenbuterol in dried blood spots | Before (baseline) as well as 3, 8, 24, 72, 168 and 240 hours after administration of clenbuterol |
| Measure | Description | Time Frame |
|---|---|---|
| Blood clenbuterol concentration | Concentration of clenbuterol in venous blood | Before (baseline) as well as 3, 8, 24, 72, 168 and 240 hours after administration of study drug |
| Urine clenbuterol concentration |
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Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| August Krogh Building | Copenhagen | Denmark |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 40946331 | Derived | Hostrup M, Moesgaard L, Fischer M, Wickham KA, Pleshardt M, Andersen AB, Bejder J, Thomassen M, Nielsen JJ, Dehnes Y, Bangsbo J, Nordsborg NB, Jessen S. Clenbuterol induces lean mass and muscle protein accretion, but attenuates cardiorespiratory fitness and desensitizes muscle beta2-adrenergic signalling. J Physiol. 2025 Oct;603(19):5529-5545. doi: 10.1113/JP289023. Epub 2025 Sep 14. |
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Two phases:
First phase sequential Second phase crossover
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First phase - open label Second phase - double-blinded
| Placebo |
| Drug |
Subjects ingest placebo tablets |
|
Concentration of clenbuterol in urine
| Before (baseline) as well as 3, 8, 24, 72, 168 and 240 hours after administration of study drug |
| Muscle strength | Maximal voluntary isometric contraction in N/m2 of the quadriceps | Before (baseline) and 2.5 hours after administration of study drug |
| Muscle signalling | Protein kinase A phosphorylation in vastus lateralis biopsies | Before (baseline) and 2.5 hours after administration of study drug |
| Plasma K+ | Venous plasma K+ concentration | Before (baseline) as well as 2.5 hours after administration of study drug |
| Muscle mTOR signalling | mTOR phosphorylation in vastus lateralis biopsies | Before (baseline) as well as 2.5 hours after administration of study drug |
| Metabolic rate | Respriatory exchange ratio of oxygen and CO2 | Before (baseline) as well as 2.5 hours after administration of study drug |
| Maximal voluntary strength | Maximal voluntary isometric muscle strength of the quadriceps | Before (baseline) and after two-week treatment of study drug |
| Body composition | Lean and fat mass during a DXA-scan | Before (baseline) and after two-week treatment of study drug |
| Aerobic capacity | Maximal oxygen uptake during maximal exercise | Before (baseline) and after two-week treatment of study drug |