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The investigators want to observe whether different doses of pancreatic polypeptide infusions influence gastric accommodation (measured as intragastric pressure changes during a liquid meal infusion), gastric emptying and food intake.
As pancreatic polypeptide (PP) influences food behavior in humans, our research group suggests a role via the the gastric accommodation or gastric emptying. Twelve healthy volunteers participated in this single blind, placebo-controlled, crossover trial. An infusion catheter and a manometry probe were positioned in the stomach. After a 15 min stabilization period, saline (placebo), PP 3 pmol/kg*min or PP 10 pmol/kg*min were intravenously infused. Thirty min after the condition, an intragastric nutrient drink (ND) infusion (60 ml/min) started until the volunteer felt maximal satiated. GE was evaluated using the 13C breath test during the 6 hours following the ND infusion. Satiation and hunger were scored on a visual analog scale every 5 minutes.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| 3 pmol/kg*min pancreatic polypeptide | Experimental | 3 pmol/kg*min of pancreatic polypeptide was intravenously infused. |
|
| 10 pmol/kg*min pancreatic polypeptide | Experimental | 10 pmol/kg*min of pancreatic polypeptide was intravenously infused. |
|
| Placebo | Placebo Comparator | 0.9 % saline solution was intravenously infused. |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| 3 pmol/kg*min pancreatic polypeptide | Drug | PP 3 pmol/kg*min was intravenously infused 30 minutes before the meal until the end of the meal. For this purpose, a cannula was inserted into the subjects' forearm vein. Human PP (CS Bio, Menlo Park, USA) was dissolved in a 0.9 % saline solution containing 5 % albumin to reduce absorption of PP to the syringe and tubing. |
| Measure | Description | Time Frame |
|---|---|---|
| The effect of different doses of pancreatic polypeptide infusion on the gastric accommodation (intragastric pressure drop) after nutrient drink infusion. | Intragastric pressure measurements were assessed using a 36-channel high-resolution solid-state manometry probe. After nutrient drink infusion, the intragastric pressure drops to a minimum value (Nadir), which is a measurement for gastric accommodation, and the pressure restores after. The time point of reaching the Nadir is different between subjects, but pressure is measured until 2 hours after nutrient drink to assess the drop. | Until 2 hours after the start of the liquid meal. Liquid meal started 30 minutes after pancreatic peptide dose or placebo iv infusion. |
| Measure | Description | Time Frame |
|---|---|---|
| The effect of different doses of pancreatic polypeptide infusion on nutrient tolerance. | Liquid nutrient drink was infused at a constant speed of 60 ml/min. At 1-min intervals subjects were asked to score their satiation using a graphic rating scale that combines verbal descriptors on a scale graded from 0 to 5 (0 is threshold, 5 is maximal satiation. Infusion stopped when the subject reached maximal satiation. The time of infusion is a measurement of their nutrient tolerance. |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Affiliation | Role |
|---|---|---|
| Jan Tack | UZ Leuven | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Jan Tack | Leuven | 3000 | Belgium |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 34435472 | Derived | Verbeure W, Rotondo A, Janssen P, Carbone F, Tack J. Supraphysiological effects of pancreatic polypeptide on gastric motor function and nutrient tolerance in humans. Physiol Rep. 2021 Sep;9(17):e15002. doi: 10.14814/phy2.15002. |
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| ID | Term |
|---|---|
| D010191 | Pancreatic Polypeptide |
| ID | Term |
|---|---|
| D010187 | Pancreatic Hormones |
| D036361 | Peptide Hormones |
| D006728 | Hormones |
| D006730 | Hormones, Hormone Substitutes, and Hormone Antagonists |
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| 10 pmol/kg*min pancreatic polypeptide | Drug | PP 10 pmol/kg*min was intravenously infused 30 minutes before the meal until the end of the meal. For this purpose, a cannula was inserted into the subjects' forearm vein. Human PP (CS Bio, Menlo Park, USA) was dissolved in a 0.9 % saline solution containing 5 % albumin to reduce absorption of PP to the syringe and tubing. |
|
|
| Placebo | Drug | Placebo (saline solution) was intravenously infused 30 minutes before the meal until the end of the meal. For this purpose, a cannula was inserted into the subjects' forearm vein. The placebo consists of a 0.9 % saline solution containing 5 % albumin. |
|
| Infusion of the liquid meal ends after 20 minutes or earlier if max satiation is reached by the subjects. |
| The effect of different doses of pancreatic polypeptide infusion on gastric emptying rate. | Gastric emptying rate after placebo and PP 10 pmol/kg*min administration was quantified using the breath test. 13C-labeled sodium octanoate were added to the ND (200 mg/L) and emptying of the stomach was assessed by analysis of the exhaled 13CO2. | Breath samples were collected in exetainers, twice before and every 15 min after the meal until 6 hours thereafter. |
| The effect of different doses of pancreatic polypeptide infusion on satiety and return of hunger. | The subjects rate their feeling of satiety (in fasted state)/satiation (in fed state) and hunger using a 100 mm line ranging from 0 to 100, as a response on the questions: 'How hungry do you feel? ' and 'How satisfied do you feel?'. 0 means 'not at all', and 100 means 'extremely much'. These feelings cannot be considered to have a better or worse outcome. One expect to have higher hunger and lower satiety scores in fasted state and lower hunger and higher satiation scores in the fed state. | Satiety and hunger were scored twice before and every 15 min after the meal until 6 hours thereafter. |
| D009479 | Neuropeptides |
| D010455 | Peptides |
| D000602 | Amino Acids, Peptides, and Proteins |
| D009419 | Nerve Tissue Proteins |
| D011506 | Proteins |