Assisted Hatching in Frozen Blastocysts

The Effect of Assisted Hatching on Implantation Rate in Frozen Blastocyst Transfer - a Prospective Randomized Controlled Study

The current study aims to assess the effect of assisted hatching on the implantation rate of frozen blastocytes.

The Zona Pellucida protects the ovum from the penetration of multiple sperm, and from mechanical and physiological damage until the fertilized ovum reaches the uterus. Subsequently, spontaneous hatching occurs, and enables the embryo to attach to the endometrium. The technique of assisted hatching was originally investigated as a means to improve implantation in cases of zona hardening, and is commonly performed in 3-day embryos. In the current study, the investigators aim to investigate the effect of assisted hatching on 5-day frozen embryos.

Inclusion Criteria:

• In-vitro fertilization patients at the investigators' institution, intended to undergo a transfer of a frozen five-day embryo (blastocyte)
• 18 to 39 years old
• First to third treatment cycle
• Patient has previously had a maximum of four embryos transferred.

Exclusion Criteria:

• Over 40 years old
• Congenital or acquired uterine malformations
• Hydrosalpinx
• Chronic autoimmune diseases
• Embryo intended to be undergo preimplantation genetic diagnosis