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PIRAMIDE study design will test the hypothesis that simultaneous interactions between DNA methylation and microRNAs may hit T2D candidate genes and predict the development of T2D-related cardiovascular complications.
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| Measure | Description | Time Frame |
|---|---|---|
| DNA methylation status both of CD4+ and CD8+ cells | Using Methylation 27K BeadChip platform based on Bisulfite conversion technology, DNA methylation profile of 35 preselected T2D patients will be measured both in CD4+ and CD8+ cells collected from peripheral blood respect with controls. | 3 Months |
| Bioinformatics analysis to predict putative novel T2D candidate genes | The network-based algorithm "Weighted Human DNA methylation PPI network (WMPN)" will be applied to methylome data in order to obtain a disease module containing the crucial differentially methylated genes both in CD4+ and CD8+ cells from T2D patients and controls. | 6 Months |
| RNA sequencing analysis both in CD4+ and CD8+ cells | RNA sequencing analysis by using Illumina HiSeq2000 Next Generatin Sequencing (NGS) platform will be performed for identifying differentially expressed micro-RNA and mRNA target both in CD4+ and CD8+ cells isolated from T2D patients and controls . | 3 Months |
| Measure | Description | Time Frame |
|---|---|---|
| ROC curves to evaluate putative DNA methylation/microRNA interactions as prognostic biomarkers of cardiovascular dysfunction in T2D patients | The ROC curves will be used to correlation putative epigenetics interactions (DNA methylation and microRNA) with the presence or absence of T2D-related cardiovascular dysfunction. | 12 Months |
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Exclusion Criteria:
Exclusion Criteria:
• Patients with known history of cancer, malignancy disorders, active infections, and chronic or immune-mediated diseases.
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We will enroll 35 men and women 50 years or older with diagnosis of T2D and 35 sex and age-matched controls. Population study will be recruited at the Department of Medical, Surgical, Neurological, Metabolic and Geriatric Sciences of University della Campania "Luigi Vanvitelli".
| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Giuditta Benincasa, MSc | Contact | +390815667916 | giuditta.benincasa@unicampania.it | |
| Claudio Napoli, Professor | Contact |
| Name | Affiliation | Role |
|---|---|---|
| Giuditta Benincasa, MSc | Clinical Department of Internal Medicine and Specialistics | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Università della Campania Luigi Vanvitelli | Recruiting | Naples | Italy |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 33194177 | Derived | Benincasa G, Franzese M, Schiano C, Marfella R, Miceli M, Infante T, Sardu C, Zanfardino M, Affinito O, Mansueto G, Sommese L, Nicoletti GF, Salvatore M, Paolisso G, Napoli C. DNA methylation profiling of CD04+/CD08+ T cells reveals pathogenic mechanisms in increasing hyperglycemia: PIRAMIDE pilot study. Ann Med Surg (Lond). 2020 Oct 20;60:218-226. doi: 10.1016/j.amsu.2020.10.016. eCollection 2020 Dec. |
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| ID | Term |
|---|---|
| D003924 | Diabetes Mellitus, Type 2 |
| D002318 | Cardiovascular Diseases |
| D003920 | Diabetes Mellitus |
| ID | Term |
|---|---|
| D044882 | Glucose Metabolism Disorders |
| D008659 | Metabolic Diseases |
| D009750 | Nutritional and Metabolic Diseases |
| D004700 | Endocrine System Diseases |
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Peripheral blood CD4+ T cells and CD8+ T cells