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| Name | Class |
|---|---|
| University Ghent | OTHER |
| Belgian Federation Against Cancer | OTHER |
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The goal of this project is to investigate the extent and role of mesothelial - mesenchymal transition (MMT) and cancer associated fibroblasts (CAFs) in the pathogenesis of colorectal peritoneal carcinomatosis (PC).
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Cytoreductive surgery (CRS) | In patients presenting with colorectal peritoneal carcinomatosis, peritoneal tissue will be sampled during surgery at 4 different locations. |
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| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Sampling peritoneal tissue | Procedure | Resection specimen will be obtained during CRS from normal peritoneum at a distance, normal peritoneum close to a peritoneal metastasis, miliary peritoneal carcinomatosis, and established peritoneal carcinomatosis. |
| Measure | Description | Time Frame |
|---|---|---|
| Immunohistochemistry (IHC) analysis | Extensive IHC analysis will be performed including CD44, integrins, ICAM-1, hyaluronate, and VCAM-1 (adhesion molecules); calretinin, mesothelin, WT1, cytokeratins and E-cadherin (mesothelial markers); α-SMA, FAP and podoplanin (CAF specific markers); PDGF, VEGF and EDGF (angiogenesis related markers) | Within 6 months after collection of the samples |
| Measure | Description | Time Frame |
|---|---|---|
| Intra-tumoral versus peritoneal vascularity | Vacularity will be assed using chalkley counts | Within 6 months after collection of the samples |
| Laser capture microdisssection (LCM) followed by gene expression analysis |
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Inclusion Criteria:
• Patients presenting with colorectal peritoneal carcinomatosis
Exclusion Criteria:
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Patients presenting with colorectal peritoneal carcinomatosis
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Ghent University Hospital | Ghent | 9000 | Belgium |
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| ID | Term |
|---|---|
| D010534 | Peritoneal Neoplasms |
| ID | Term |
|---|---|
| D000008 | Abdominal Neoplasms |
| D009371 | Neoplasms by Site |
| D009369 | Neoplasms |
| D004067 | Digestive System Neoplasms |
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Different cell types (mesothelial cells, submesothelial resident fibroblasts, CAFs) will be isolated using LCM, followed by gene expression analysis
| Within 12 months after collection of the samples |
| D004066 |
| Digestive System Diseases |
| D010532 | Peritoneal Diseases |