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A 4mm Punch biopsy is obtained from lesional and non lesional skin of 25 psoraitic patients as well as of 30 controls.
Skin biopsy from patients (psoriasis lesional and non lesional) & control will be kept in lysis solution (which contains RNAase inhibitor) for the stability of the parameter to be assessed. ERAP-1 gene expression will Research Template 5 Final Version: 8/3/2 016 be detected by quantitative real time -PCR, which include RNA extraction, cDNA synthesis & PCR.
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| cases of psoriasis | Experimental | group of 25 cases of psoriasis subjected to skin biopsy for detection of lesional and non lesional expression of ERAP1 by RT-PCR |
|
| normal controls | Experimental | group of 30 age and sex matched healthy controls o subjected to skin biopsy from normal skin for detection of expression of ERAP1 by RT-PCR |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| RT-PCR for ERAP1 detection | Diagnostic Test | Reverse transcriptase -Polymerase Chain Reaction for ERAP1 detection |
|
| Measure | Description | Time Frame |
|---|---|---|
| Estimation of lesional ERAP1 in psoriatic skin | DPCRetection of level of expression of ERAP 1 in lesional skin of psoriasis patients by RT- | Maximum 2 years |
| Measure | Description | Time Frame |
|---|---|---|
| Estimation of non lesional ERAP1 in psoriatic skin | Detection of level of expression of ERAP 1 in non lesional skin of psoriasis patients by RT-PCR. | Maximum 2 years |
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Inclusion Criteria:
Exclusion Criteria:
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| ID | Term |
|---|---|
| D011565 | Psoriasis |
| ID | Term |
|---|---|
| D017444 | Skin Diseases, Papulosquamous |
| D012871 | Skin Diseases |
| D017437 | Skin and Connective Tissue Diseases |
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