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This project is expected to confirm the hypothesis that hyposplenism in patients with celiac disease is not conditioned by a selective memory deficiency of B lymphocytes.
Other objectives of project are:
Ultrasound examination is focused to spleen size (spleen volumetry). Methodology of laboratory examinations is based on venous blood sampling for the determination of memory B-like marginal zone by flow cytometry; gliadin 33-mer (ELISA) determination in faecal and urine sample; blood plasma extraction for citrulline (high performance liquid chromatography, HPLC).
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Patients | Experimental | Ultrasound examination: scaled to spleen size (spleen volumetry) Methodology of laboratory tests: venous blood sampling |
|
| healthy blood donors | No Intervention | No intervention in this group. |
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| venous blood sampling | Diagnostic Test | Venous blood samples to determine memory B lymphocytes. |
| |
| Measure | Description | Time Frame |
|---|---|---|
| concentration of B-lymphocytes as parameter to evaluate treatment effect | assessment method: blood sampling, units: the measurement results are in absolute numbers, method: flow cytometry | one year |
| concentration of B-lymphocytes as parameter to evaluate treatment effect | assessment method: blood sampling, units: the measurement results are in percentage numbers, method: flow cytometry | one year |
| concentration of citrullin in plasma as parameter to evaluate treatment effect | assessment method: blood sampling, units: μmol/l (micromol/l; Citrullin cut-off for intestinal failure is <20μmol / L), method: mass spectrometry (LC-MS instrumentation with internal isotopically labeled standard) | one year |
| concentration of ornithin in plasma as parameter to evaluate treatment effect | assessment method: blood sampling, units: μmol/l (micromol/l), method: mass spectrometry (LC-MS instrumentation with internal isotopically labeled standard) | one year |
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Inclusion Criteria:
Exclusion Criteria:
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| Name | Role | Phone | Extension | |
|---|---|---|---|---|
| Ladislav Douda, M.D. | Contact | 495 83 4240 | ladislav.douda@fnhk.cz |
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| University Hospital Hradec Králové | Recruiting | Hradec Králové | The Czech Republic | 500 05 | Czechia |
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| Label | URL |
|---|---|
| Related Info | View source |
| Related Info | View source |
| Related Info |
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50 patients and 30 healthy blood donor
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| spleen volumetry |
| Procedure |
Ultrasound examination of spleen to scaled the size. |
|
| Related Info | View source |
| ID | Term |
|---|---|
| D002446 | Celiac Disease |
| ID | Term |
|---|---|
| D008286 | Malabsorption Syndromes |
| D007410 | Intestinal Diseases |
| D005767 | Gastrointestinal Diseases |
| D004066 | Digestive System Diseases |
| D008659 | Metabolic Diseases |
| D009750 | Nutritional and Metabolic Diseases |
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| ID | Term |
|---|---|
| D001800 | Blood Specimen Collection |
| ID | Term |
|---|---|
| D013048 | Specimen Handling |
| D019411 | Clinical Laboratory Techniques |
| D019937 | Diagnostic Techniques and Procedures |
| D003933 | Diagnosis |
| D011677 | Punctures |
| D013514 | Surgical Procedures, Operative |
| D008919 | Investigative Techniques |
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