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| Name | Class |
|---|---|
| University of Copenhagen | OTHER |
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Successful skeletal muscle regeneration depends on a functional pool of muscle stem cells, termed satellite cells (SC). SC are in a quiescent state throughout adulthood, but undergo multiple cycles of proliferation and self-renewal in response to muscle damage. During aging, there is a loss of SC quiescence, and SC more readily enter an ageing-state impairing their function.
Animal studies have revealed a common denominator for increasing SC function and activity, namely Sirtuin activation. Natural stimulators of Sirtuins includes Nicotinamide Riboside (NR) (a Nicotinamide adenine dinucleotide (NAD+) precursor) and the polyphenol Pterostilbene (PT). In this study, we aim to investigate if NR+PT supplementation will promote skeletal muscle regeneration after muscle damage in elderly humans by enhanced recruitment of SC.
14 days after start of placebo or NR/PT (500/100 mg twice daily) supplementation a muscle damage will induced in the m. vastus lat. by electric stimulation combined with eccentric work in a dynamometer. Muscle biopsies will be collected at 2 hours, 2, 8 and 30 days post damage and placebo or NR/PT supplementation will continue for the whole study period (45 days).
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| Label | Type | Description | Intervention Names |
|---|---|---|---|
| Placebo | Placebo Comparator |
| |
| Nicotinamide Riboside + Pterostilbene | Experimental |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| Placebo (twice daily) | Other | Matching placebo |
| |
| Nicotinamide Riboside/Pterostilbene (500 mg/100 mg twice daily) |
| Measure | Description | Time Frame |
|---|---|---|
| Presence of Satellite Cells | quantified by immunohistochemistry in muscle biopsies | Change from baseline up to 45 days |
| Measure | Description | Time Frame |
|---|---|---|
| Activation of Satellite cells | determined by immunohistochemistry and FACS | Change from baseline up to 45 days |
| Presence of macrophages | determined by immunohistochemistry and FACS |
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Inclusion Criteria:
Exclusion Criteria:
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| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Aarhus University Hospital | Aarhus N | 8200 | Denmark |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 35998039 | Derived | Jensen JB, Dollerup OL, Moller AB, Billeskov TB, Dalbram E, Chubanava S, Damgaard MV, Dellinger RW, Trost K, Moritz T, Ringgaard S, Moller N, Treebak JT, Farup J, Jessen N. A randomized placebo-controlled trial of nicotinamide riboside and pterostilbene supplementation in experimental muscle injury in elderly individuals. JCI Insight. 2022 Oct 10;7(19):e158314. doi: 10.1172/jci.insight.158314. |
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| ID | Term |
|---|---|
| C018613 | nicotinamide-beta-riboside |
| C000721168 | Pterocarpus marsupium |
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| Other |
Elysium Basis TM, Elysium Health, Inc., NY, USA |
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| Change from baseline up to 45 days |
| Presence of Fibro/Adipogenic Progenitors | quantified by FACS | Change from baseline up to 45 days |
| Presence of damaged muscle fibers | determined by immunohistochemistry | Change from baseline up to 45 days |
| Presence of muscle regenerative fibers | determined by immunohistochemistry | Change from baseline up to 45 days |
| Presence of autophagy in relation to muscle damage | determined by Western blot and PCR | Change from baseline up to 45 days |
| Lipid accumulation in skeletal muscle tissue and liver | determined by magnetic resonance spectroscopy | Change from baseline to 8 days |
| Blood glucose response in relation to muscle damage | determined by Continuous glucose monitoring | Change from baseline to 8 days |
| Body composition (lean body mass and fat body mass) | measured by Dual energy X-ray absorptiometry | Change from baseline to 45 days |
| Muscle strength | determined from maximal voluntary contraction of the m. quadriceps femoris | Change from baseline up to 45 days |