In order to further enable physiopathology studies, the aim of this project is to validate an in vitro model of cystic fibrosis created using induced pluripotent stemcell (iPS) differentiated bronchial epithelium from cystic fibrosis (CF) patients homozygous for the p.Phe508del mutation of the cystic fibrosis transmembrane conductance regulator (CFTR) gene.

The investigator's primary objective is to generate iPS lines from 3 CF patients and from 3 healthy subjects.

Secondary objectives include verification that cell lines express the CFTR gene according to their genotype, verification or relative production of the CFTR protein for each iPS line, and amplification of obtain iPS lines for aliquot creation to facilitate sharing.

Inclusion Criteria for subjects with Cystic Fibrosis:

• Homozygote for the p.Phe508del mutation
• Signed informed consent given by the subject

Inclusion Criteria for subjects without Cystic Fibrosis:

• Signed informed consent given by the subject

Exclusion Criteria:

• Pregnancy, breastfeeding
• Participant in an exclusion period determined by a previous study
• Participant under any kind of guardianship
• Unaffiliated with or not a beneficiary of a social security program (health insurance)
• Subject deprived of liberty (e.g. prisoners)
• Subject with positive infectious markers for HIV1, HIV2, HBC or HBV

Exclusion Criteria for subjects with Cystic Fibrosis:

• Any pathology requiring a treatment or a pathology not requiring treatment but with clinical significance according to the investigator

Three cystic fibrosis patients who are homozygous for the p.Phe508del mutation and 3 healthy subjects without cystic fibrosis.