Does Hyaluronic Acid Affect Periodontal Treatment?

Evaluation of Clinical and Biochemical Effects of Hyaluronic Acid on Periodontitis: A Split-mouth Randomized-controlled Trial

Objective: The aim of this study is to evaluate the effect of HA as an adjunct to scaling and root planning (SRP) on clinical parameters, periodontal inflamed surface area (PISA) and adenosine deaminase (ADA), catalase (CAT), and glutathione (GSH) levels in gingival crevicular fluid (GCF) in patients with periodontitis.

Design: A total of 24 Stage II-III periodontitis patients will be included in this split-mouth, randomized-controlled study. The study population will be divided into four groups: in Group 1 (SRP): SRP+ saline; in Group 2 (SRP+HAgel): SRP + HA gel; in Group 3 (SRP+HAmo): SRP+ HA mouthrinse; and in Group 4 (SRP+HAmo+HAgel): SRP+HA mouthrinse + HA gingival gel were applied. At baseline and Week 4 periodontal clinical parameters and PISA will be calculated. Also, GCF amount will be collected to evaluate ADA, CAT, and GSH by spectrophotometric analysis.

Inclusion Criteria:

• Systemically healthy
• moderate-to-severe periodontitis
• at least five sites with ≥5mm probing depth (PD)
• at least ≥20 teeth
• Stage II-III periodontitis according to the 2017 World Workshop
• Age between18-55

Exclusion Criteria:

• receiving systemic or local antibiotics within the past six months
• prior surgical or non-surgical periodontal treatment within the past six months
• smoking
• pregnancy
• lactation
• age ≤18 years or ≥55 years
• hypertension
• known chronic systemic diseases (i.e., diabetes mellitus, rheumatoid arthritis), - - known hypersensitivity to one of the ingredients of the HA preparation.