Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
COVID-19
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
To elucidate the longitudinal development of intestinal microbiota in patients with IL10RA deficiency after hematopoietic stem cell transplantation (HSCT). The investigators planned to collect fecal samples from IL10RA-deficient patients who received HSCT. Samples were collected more than once every three days after engraftment in lamina flow ward and at least once a week before discharge. Microbial DNA was extracted from the fecal samples. And all analysis was based on the next generation sequencing data.
Hematopoietic stem cell transplantation is considered the only curative therapy for patients with interleukin-10 receptor-A(IL10RA) deficiency. The investigators aimed to collect the fecal samples of these patients throughout the conditioning, transplantation until discharge, providing a dense insight into the longitudinal development of intestinal microbiota.
Not provided
Not provided
Not provided
Not provided
Not provided
| Label | Type | Description | Intervention Names |
|---|---|---|---|
| HSCT cohort | IL10RA-deficient patients who received hematopoietic stem cell transplantation |
|
| Name | Type | Description | Arm Group Labels | Other Names |
|---|---|---|---|---|
| hematopoietic stem cell transplantation | Procedure |
|
| Measure | Description | Time Frame |
|---|---|---|
| Shannon indices | Shannon index is a popular diversity index in the ecological literature; minimum score is zero, higher values represent a better outcome | during the hospitalization of each eligible patient, an average of 90 days per patient |
| Simpson indices | Simpson index in ecology is to measure the degree of concentration when individuals are classified into types; scale ranges [0,1]; lower values represent a better outcome | during the hospitalization of each eligible patient, an average of 90 days per patient |
| relative abundance of Proteobacteria | describe the microbiome at the phylum level | during the hospitalization of each eligible patient, an average of 90 days per patient |
| relative abundance of Firmicutes | describe the microbiome at the phylum level | during the hospitalization of each eligible patient, an average of 90 days per patient |
| Clostridium sensus_stricto_1 | describe the microbiome at the genus level | during the hospitalization of each eligible patient, an average of 90 days per patient |
| Escherichia - Shigella | describe the microbiome at the genus level | during the hospitalization of each eligible patient, an average of 90 days per patient |
| Measure | Description | Time Frame |
|---|---|---|
| chimerism | The level of chimerism of each patient will be reported as percentages [0,100%] | during the hospitalization of each eligible patient, an average of 90 days per patient |
| manifestation of graft-versus-host disease |
| Measure | Description | Time Frame |
|---|---|---|
| relative abundance of Clostridiales | describe the microbiome at the order level | during the hospitalization of each eligible patient, an average of 90 days per patient |
| Shannoneven indices | measure the community evenness, scale ranges [0,1]; higher values represent a better outcome |
Inclusion Criteria:
Exclusion Criteria:
Not provided
Not provided
Patients who were diagnosed with IL10RA deficiency and who received the hematopoietic stem cell transplantation in a tertiary hospital were enrolled.
Not provided
| Name | Affiliation | Role |
|---|---|---|
| Ying Huang, MD | Children's Hospital of Fudan University | Principal Investigator |
| Facility | Status | City | State | ZIP | Country | Contacts |
|---|---|---|---|---|---|---|
| Children's hospital of Fudan university | Shanghai | Shanghai Municipality | 201102 | China |
| PubMed Identifier | Type | Citation | Retractions |
|---|---|---|---|
| 29788474 | Result | Peng K, Qian X, Huang Z, Lu J, Wang Y, Zhou Y, Wang H, Wu B, Wang Y, Chen L, Zhai X, Huang Y. Umbilical Cord Blood Transplantation Corrects Very Early-Onset Inflammatory Bowel Disease in Chinese Patients With IL10RA-Associated Immune Deficiency. Inflamm Bowel Dis. 2018 Jun 8;24(7):1416-1427. doi: 10.1093/ibd/izy028. |
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
Not provided
| ID | Term |
|---|---|
| D015212 | Inflammatory Bowel Diseases |
| C567728 | Inflammatory Bowel Disease 28, Autosomal Recessive |
| ID | Term |
|---|---|
| D005759 | Gastroenteritis |
| D005767 | Gastrointestinal Diseases |
| D004066 | Digestive System Diseases |
| D007410 | Intestinal Diseases |
Not provided
Not provided
| ID | Term |
|---|---|
| D018380 | Hematopoietic Stem Cell Transplantation |
| ID | Term |
|---|---|
| D033581 | Stem Cell Transplantation |
| D017690 | Cell Transplantation |
| D064987 | Cell- and Tissue-Based Therapy |
| D001691 | Biological Therapy |
Not provided
Not provided
Not provided
Not provided
Not provided
Fecal samples
|
describe the graft-versus-host disease happened to each patient
| during the hospitalization of each eligible patient, an average of 90 days per patient |
| Sobs indices | describe the the observed richness of each sample | during the hospitalization of each eligible patient, an average of 90 days per patient |
| Chao1 estimator | describe the operational taxonomic unit(OTU) numbers of each sample | during the hospitalization of each eligible patient, an average of 90 days per patient |
| neutrophil cell count | blood test results | during the hospitalization of each eligible patient, an average of 90 days per patient |
| platelet count | blood test results | during the hospitalization of each eligible patient, an average of 90 days per patient |
| during the hospitalization of each eligible patient, an average of 90 days per patient |
| Simpsoneven indices | measure community evenness, scale ranges [0,1]; higher values represent a better outcome | during the hospitalization of each eligible patient, an average of 90 days per patient |
| D013812 | Therapeutics |
| D014180 | Transplantation |
| D013514 | Surgical Procedures, Operative |